Human RGM-B Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human RGM-B in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse RGM-B is observed and less than 1% cross-reactivity with recombinant human (rh) RGM-A and rhRGM-C is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human RGM-B isoform 1
    Gly46-SerSer411
    Accession # Q6NW40
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human RGM-B (Catalog # 3630-RG)
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of RGM‑B in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Goat Anti-Human RGM‑B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3630, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody
(Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RGM-B

RGM-B, also known as DRAGON, is a 40 kDa member of the repulsive guidance molecule (RGM) family of GPI-linked neuronal and muscle membrane proteins (1, 2). It is synthesized as a preproprotein that consists of a 45 amino acid (aa) signal sequence, a 368 aa mature region, and a 24 aa C-terminal prosegment (3). RGM-B contains an RGD motif, two potential N-linked glycosylation sites, and an abbreviated von Willebrand factor domain. There is a potential proteolytic cleavage site within the VWF domain (4). Alternative splicing may generate isoforms of RGM-B with N-terminal extensions or truncation following the VWF domain. Mature human RGM-B shares 52% and 36% aa sequence identity with the comparable regions of RGM-A and RGM-C, respectively. It shares 98%, 92%, 92%, and 78% aa sequence identity with macaque, mouse, bovine, and chicken RGM-B, respectively. RGM-B is expressed in the developing and adult nervous system, particularly in the dorsal root ganglia and mantle layer of the spinal cord (3‑5). In mouse, it shows a complementary, non-overlapping distribution with RGM-A (2‑5). RGM-B is also expressed in fetal and adult enteric ganglia and in postnatal intestinal epithelium (6). RGM-B expression has been detected in neuronal cell bodies and proximal axonal segments (4) but is also present on the cell surface, where it interacts homophilically and mediates neuronal adhesion (3). RGM-B additionally functions as a BMP coreceptor. It directly binds BMP-2 and -4 but not other TGF-beta family proteins (7). RGM-B associates with BMP type I (ALK-2, -3, -6) and type II (Activin RIIA, Activin RIIB) receptors and enhances BMP signaling (7).

  • References:
    1. Monnier, P.P. et al. (2002) Nature 419:392.
    2. Schmidtmer, J. and D. Engelkamp (2004) Gene Exp. Patterns 4:105.
    3. Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
    4. Niederkofler, V. et al. (2004) J. Neurosci. 24:808.
    5. Oldekamp, J. et al. (2004) Gene Exp. Patterns 4:283.
    6. Metzger, M. et al. (2005) Dev. Dyn. 234:169.
    7. Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.
  • Long Name:
    Repulsive Guidance Molecule B
  • Entrez Gene IDs:
    285704 (Human); 68799 (Mouse)
  • Alternate Names:
    DKFZp434P228; Dragon; DRG11-responsive axonal guidance and outgrowth of neurite; RGM domain family, member B; RGMB; RGM-B
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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