Human RIG-I Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human RIG-I in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human RIG-I
    Met724-Lys925
    Accession # O95786
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human RIG-I by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line and Nalm‑6 human Pre‑B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RIG-I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4859) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RIG-I at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RIG-I

RIG-I (Retinoic-acid inducible gene I; also Dead-box protein 58) is a 100 kDa member of the DexH-box family of proteins. It is expressed in smooth muscle cells and endothelium. RIG-I is normally an inactive monomer. Upon Sendai and Hepatitis C virus infection, RIG-I binds to dsRNA, initiating homodimerization and downstream activation of IRF3 and KF kappa B genes. Human RIG-I is 925 amino acids (aa) in length. It contains two CARD domains (aa 1‑172), one helicase ATP-binding region (aa 251‑430), and one C-terminal repressor domain (aa 735‑925). There are three potential splice variants. One shows an alternate start site at Met204, while another shows deletion of aa 3‑80, and a third shows a deletion of aa 36‑80 plus 749‑925. Over aa 724‑925, human RIG-I is 76% aa identical to mouse RIG-I.

  • Long Name:
    RIG-I
  • Entrez Gene IDs:
    23586 (Human); 230073 (Mouse)
  • Alternate Names:
    DDX58; DEAD (Asp-Glu-Ala-Asp) box polypeptide 58; DEAD box protein 58; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; DKFZp686N19181; EC 3.6.4.13; FLJ13599; probable ATP-dependent RNA helicase DDX58; retinoic acid inducible gene I; Retinoic acid-inducible gene 1 protein; Retinoic acid-inducible gene I protein; RIG-1; RIGI; RIG-I; RIG-IDKFZp434J1111; RNA helicase RIG-I
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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