Human RIPK3/RIP3 Antibody (1312D)

R&D Systems | Catalog # MAB76042

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1312D
View all RIPK3/RIP3 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human RIPK3/RIP3
Accession # Q9Y572

Specificity

Detects human RIPK3/RIP3 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human RIPK3/RIP3 Antibody (1312D)

Detection of Human RIPK3/RIP3 antibody by Western Blot.

Detection of Human RIPK3/RIP3 by Western Blot.

Western blot shows lysates of SK-BR-3 human breast cancer cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human RIPK3/RIP3 Monoclonal Antibody (Catalog # MAB76042) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for RIPK3/RIP3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
RIPK3/RIP3 antibody in SK-BR-3 and HepG2 Human Cell Lines by Immunocytochemistry (ICC).

RIPK3/RIP3 in SK-BR-3 and HepG2 Human Cell Lines.

RIPK3/RIP3 was detected in immersion fixed SK-BR-3 human breast cancer cell line (positive stain) and HepG2 human hepatocellular carcinoma cell line (negative stain) using Rabbit Anti-Human RIPK3/RIP3 Monoclonal Antibody (Catalog # MAB76042) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human RIPK3/RIP3 Antibody (1312D)

Application
Recommended Usage

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed SK-BR-3 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line

Western Blot

2 µg/mL
Sample: SK‑BR‑3 human breast cancer cell line and Raji human Burkitt's lymphoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB76042 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RIPK3/RIP3

RIP3 (Receptor-Interacting Protein 3), also known as RIPK3, is a 518 amino acid (aa), 60 kDa phosphoprotein that contains an N-terminal protein kinase domain and a C-terminal interaction motif with which it binds RIP1. It converts cell response to TNF-a from apoptosis (RIP1 via Fas) to programmed necrosis (RIP1/RIP3) by inducing reactive oxygen species production. Human RIP3 (aa 1-218) shares approximately 74% aa sequence identity with mouse and rat RIP3. Alternately spliced human b (252 aa) and g (231 aa) isoforms diverge after aa 219 and 221, respectively, and are found to antagonize RIP3.

Long Name

Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 3

Alternate Names

RIP3

Entrez Gene IDs

11035 (Human); 56532 (Mouse); 246240 (Rat)

Gene Symbol

RIPK3

UniProt

Q9Y572 (Human)

Additional RIPK3/RIP3 Products

Product Documents for Human RIPK3/RIP3 Antibody (1312D)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human RIPK3/RIP3 Antibody (1312D)

For research use only

Related Research Areas

Apoptosis Adaptor Proteins
NFkB Pathway

Customer Reviews for Human RIPK3/RIP3 Antibody (1312D) (1)

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  • Human RIPK3/RIP3 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult splenocytes
    Species: Human
    Verified Customer | Posted 01/17/2022
    Human RIPK3/RIP3 Antibody (1312D) MAB76042

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Protocols

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