Human RIPK3/RIP3 Antibody

Catalog # Availability Size / Price Qty
Detection of Human RIPK3/RIP3 by Western Blot.
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Product Details
Citations (3)
Supplemental Products
Reviews (1)

Human RIPK3/RIP3 Antibody Summary

Species Reactivity
Detects human RIPK3/RIP3 in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 780115
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human RIPK3/RIP3
Accession # Q9Y572
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human RIPK3/RIP3 antibody by Western Blot. View Larger

Detection of Human RIPK3/RIP3 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human RIPK3/RIP3 Monoclonal Antibody (Catalog # MAB7604) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RIPK3/RIP3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry RIPK3/RIP3 antibody in Human Spleen by Immunohistochemistry (IHC-P). View Larger

RIPK3/RIP3 in Human Spleen. RIPK3/RIP3 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human RIPK3/RIP3 Monoclonal Antibody (Catalog # MAB7604) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of splenocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RIPK3/RIP3

RIP3 (Receptor-Interacting Protein 3), also known as RIPK3, is a 518 amino acid (aa), 60 kDa phosphoprotein that contains an N-terminal protein kinase domain and a C-terminal interaction motif with which it binds RIP1. It converts cell response to TNF-a from apoptosis (RIP1 via Fas) to programmed necrosis (RIP1/RIP3) by inducing reactive oxygen species production. Human RIP3 (aa 1-218) shares approximately 74% aa sequence identity with mouse and rat RIP3. Alternately spliced human b (252 aa) and g (231 aa) isoforms diverge after aa 219 and 221, respectively, and are found to antagonize RIP3.

Long Name
Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 3
Entrez Gene IDs
11035 (Human); 56532 (Mouse); 246240 (Rat)
Alternate Names
EC; Receptor Interacting Serine/Threonine Kinase 3; Receptor-interacting protein 3; receptor-interacting serine/threonine-protein kinase 3; receptor-interacting serine-threonine kinase 3; RIP3; RIP-3; RIPK3; RIP-like protein kinase 3

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Citations for Human RIPK3/RIP3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis
    Authors: YA Kung, HJ Chiang, ML Li, YN Gong, HP Chiu, CT Hung, PN Huang, SY Huang, PY Wang, TA Hsu, G Brewer, SR Shih
    MBio, 2022;0(0):e0271721.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. ABT?737, a Bcl?2 family inhibitor, has a synergistic effect with apoptosis by inducing urothelial carcinoma cell necroptosis
    Authors: R Cheng, X Liu, Z Wang, K Tang
    Molecular Medicine Reports, 2021;23(6):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Co-Immunoprecipitation
  3. Prognostic Significance of CHIP and RIPK3 in Non-Small Cell Lung Cancer
    Authors: J Kim, JY Chung, YS Park, SJ Jang, HR Kim, CM Choi, JS Song
    Cancers (Basel), 2020;12(6):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC


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Human RIPK3/RIP3 Antibody
By Anonymous on 10/18/2021
Application: IHC Sample Tested: Nervous tissue Species: Human