Human ROR2 Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human ROR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human ROR1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human ROR2
    Val28-Gly403
    Accession # AAA60276
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human ROR2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and T47D human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human ROR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2064) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ROR2 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human ROR2 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ROR2 at approximately 139 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ROR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2064) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ROR2
ROR2 is a type I receptor tyrosine kinase important in chondrocyte, cartilage and growth plate formation.
  • Long Name:
    Receptor Tyrosine Kinase-like Orphan Receptor 2
  • Entrez Gene IDs:
    4920 (Human); 26564 (Mouse)
  • Alternate Names:
    BDB; BDB1; EC 2.7.10.1; MGC163394; neurotrophic tyrosine kinase receptor-related 2; Neurotrophic tyrosine kinase, receptor-related 2; NTRKR2; NTRKR2tyrosine-protein kinase transmembrane receptor ROR2; receptor tyrosine kinase-like orphan receptor 2; ROR2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Ror family receptor tyrosine kinases regulate the maintenance of neural progenitor cells in the developing neocortex.
    J. Cell. Sci., 2102;125(0):2017-29.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Receptor tyrosine kinase-like orphan receptor 2 (Ror2) expression creates a poised state of Wnt signaling in renal cancer.
    Authors: Rasmussen N, Wright T, Brooks S, Hacker K, Debebe Z, Sendor A, Walker M, Major M, Green J, Wahl G, Rathmell W
    J Biol Chem, 2013;288(36):26301-10.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Therapeutic Potential and Challenges of Targeting Receptor Tyrosine Kinase ROR1 with Monoclonal Antibodies in B-Cell Malignancies.
    Authors: Yang J, Baskar S, Kwong KY
    PLoS ONE, 2011;6(6):e21018.
    Species: Human
    Sample Type: Recombinant Protein
    Application: ELISA Development
  4. Wnt5a/Ror2-induced upregulation of xPAPC requires xShcA.
    Authors: Feike AC, Rachor K, Gentzel M, Schambony A
    Biochem. Biophys. Res. Commun., 2010;400(4):500-6.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Type: Cell Lysates
    Application: IP
  5. Unique cell surface expression of receptor tyrosine kinase ROR1 in human B-cell chronic lymphocytic leukemia.
    Authors: Baskar S, Kwong KY, Hofer T, Levy JM, Kennedy MG, Lee E, Staudt LM, Wilson WH, Wiestner A, Rader C
    Clin. Cancer Res., 2008;14(2):396-404.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. The Wnt-5a-derived hexapeptide Foxy-5 inhibits breast cancer metastasis in vivo by targeting cell motility.
    Authors: Safholm A, Tuomela J, Rosenkvist J, Dejmek J, Harkonen P, Andersson T
    Clin. Cancer Res., 2008;14(20):6556-63.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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