Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 674035
View all S100A2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human S100A2
Met1-Pro97
Accession # P29034

Specificity

Detects human S100A2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) S100A1, A4, A6, A7, A8, A9, A10, A11, A13, A16, rhS100B, rhS100P, recombinant mouse S100A1, A4, A6, A8, A9, A10, A13, or A16 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human S100A2 Antibody

Detection of Human S100A2 antibody by Western Blot.

Detection of Human S100A2 by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human S100A2 Monoclonal Antibody (Catalog # MAB4870) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for S100A2 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human S100A2 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: A431 human epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100A2

S100A2 (also S100L and CaN19) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins.  It is expressed by multiple cell types, including keratinocytes, chondrocytes, and bronchial epithelium.  S100A2 regulates both Ca and Zn (in human) within cells, and increases p53 activity.  It forms both noncovalent and covalent homodimers, and a homotetramer under certain conditions.  Human S100A2 is 98 amino acids (aa) in length.  It contains two EF-hand motifs (aa 13-48 and 51-86) plus a calcium (aa 64-75) and zinc (aa 17-22) binding site.  There is one potential alternative splice form that shows a 40 aa insertion after Gly48.  Full-length human S100A2 shares 89% and 85% aa identity with bovine and canine S100A2, respectively

Long Name

S100 Calcium Binding Protein A2

Alternate Names

CAN19, S100L

Entrez Gene IDs

6273 (Human)

Gene Symbol

S100A2

UniProt

P29034

Additional S100A2 Products

Product Documents for Human S100A2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human S100A2 Antibody

For research use only

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