Human S100B DuoSet ELISA

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DY1820-05
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Human S100B ELISA Standard Curve
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Product Details
Procedure
Citations (11)
FAQs
Supplemental Products
Reviews (3)

Human S100B DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Range
46.9 - 3,000 pg/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant human S100 Calcium Binding Protein B (S100B). The suggested diluent is suitable for the analysis of most cell culture supernate, serum, and plasma samples. Diluents for complex matrices, such as serum, and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product.

Scientific Data

Human S100B ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: S100B

S100B belongs to the S100 subgroup of the EF-hand family of calcium binding proteins. It is a homodimer that is expressed primarily in the brain by astrocytes, oligodendrocytes and Schwann cells. S100B has multiple intracellular functions, but can also be secreted from cells to exert extracellular functions. Some of the extracellular functions of S100B may be mediated by RAGE (receptor for advanced glycation end products). Blood levels of S100B can be used to monitor the extent of brain injury and malignant melanoma.

Long Name:
S100 Calcium Binding Protein B
Entrez Gene IDs:
6285 (Human)
Alternate Names:
beta (neural); NEF; S100 beta; S100 calcium binding protein B; S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S-100 calcium-binding protein, beta chain, 10protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100; S100B; S100beta

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Block Buffer to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human S100B DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Serum Levels of S100B Protein and Myelin Basic Protein as a Potential Biomarkers of Recurrent Depressive Disorders
    Authors: Levchuk, LA;Roschina, OV;Mikhalitskaya, EV;Epimakhova, EV;Simutkin, GG;Bokhan, NA;Ivanova, SA;
    Journal of personalized medicine
    Species: Human
    Sample Types: Plasma
  2. Astrocytes mediate cell non-autonomous correction of aberrant firing in human FXS neurons
    Authors: SD Sharma, BK Reddy, R Pal, TE Ritakari, JD Cooper, BT Selvaraj, PC Kind, S Chandran, DJA Wyllie, S Chattarji
    Cell Reports, 2023-04-04;42(4):112344.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Effects of Exercise Training on Neurotrophic Factors and Blood-Brain Barrier Permeability in Young-Old and Old-Old Women
    Authors: SY Cho, HT Roh
    International Journal of Environmental Research and Public Health, 2022-12-16;19(24):.
    Species: Human
    Sample Types: Serum
  4. Carbon monoxide (CO) correlates with symptom severity, autoimmunity, and responses to probiotics treatment in a cohort of children with autism spectrum disorder (ASD): a post-hoc analysis of a randomized controlled trial
    Authors: HT Sherman, K Liu, K Kwong, ST Chan, AC Li, XJ Kong
    BMC Psychiatry, 2022-08-08;22(1):536.
    Species: Human
    Sample Types: Serum
  5. ProBDNF as an Indicator of Improvement among Women with Depressive Episodes
    Authors: W Zwoli?ska, M Skibinska, A S?opie?, M Dmitrzak-W
    Metabolites, 2022-04-16;12(4):.
    Species: Human
    Sample Types: Serum
  6. Longitudinal assessment of S100B serum levels and clinical factors in youth patients with mood disorders
    Authors: A Rajewska-R, M Dmitrzak-W, P Kapelski, N Lepczynska, J Pawlak, J Twarowska-, M Skibinska
    Scientific Reports, 2021-06-07;11(1):11973.
    Species: Human
    Sample Types: Serum
  7. Circulating Ligands of the Receptor for Advanced Glycation End Products and the Soluble Form of the Receptor Modulate Cardiovascular Cell Apoptosis in Diabetes
    Authors: JN Tsoporis, E Hatziagela, S Gupta, S Izhar, V Salpeas, A Tsiavou, AG Rigopoulos, AS Triantafyl, JC Marshall, TG Parker, IK Rizos
    Molecules, 2020-11-10;25(22):.
    Species: Human
    Sample Types: Plasma
  8. Severe Traumatic Brain Injury (TBI) Modulates the Kinetic Profile of the Inflammatory Response of Markers for Neuronal Damage
    Authors: CR Schindler, T Lustenberg, M Woschek, P Störmann, D Henrich, P Radermache, I Marzi
    J Clin Med, 2020-06-01;9(6):.
    Species: Human
    Sample Types: Serum
  9. Chronic Food Antigen-specific IgG-mediated Hypersensitivity Reaction as A Risk Factor for Adolescent Depressive Disorder
    Authors: R Tao, Z Fu, L Xiao
    Genomics Proteomics Bioinformatics, 2019-06-21;0(0):.
    Species: Human
    Sample Types: Serum
  10. Effects of Kudiezi Injection on Serum Inflammatory Biomarkers in Patients with Acute Cerebral Infarction
    Authors: X Liu, X Jin, B Chen, X Liu, X Liang, X Fang, H Wu, X Fu, H Zheng, X Ding, N Duan, Y Zhang
    Dis. Markers, 2018-09-02;2018(0):7936736.
    Species: Human
    Sample Types: Serum
  11. Cognitive dysfunction correlates with elevated serum S100B concentration in drug-free acutely relapsed patients with schizophrenia
    Authors: Yunlong Tan
    Psychiatry Res, 2016-09-22;247(0):6-11.
    Species: Human
    Sample Types: Serum

FAQs

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Reviews for Human S100B DuoSet ELISA

Average Rating: 4.3 (Based on 3 Reviews)

5 Star
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Human S100B DuoSet ELISA
By James Williams on 03/16/2022
Sample Tested: EDTA Plasma

Protocol was clear and standards worked, however we weren't able to get detectable levels in plasma


Human S100B DuoSet ELISA
By Anonymous on 11/12/2020
Sample Tested: EDTA Plasma

Samples applied neat, consistent replicates


Human S100B DuoSet ELISA
By Anonymous on 01/15/2020
Sample Tested: Purified Standard