Detection of Human SALM4/LRFN3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SALM4/LRFN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5349) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SALM4/LRFN3 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Synaptic adhesion-like molecule 4 (SALM4; also leucine-rich repeat and fibronectin type-III domain-containing protein 3 (Lrfn3) is an approximately 90 kDa member of the Lrfn family of type I transmembrane glycoproteins (1). Human SALM4 is synthesized as a 628 amino acid (aa) precursor that contains a 16 aa signal sequence, a 523 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 68 aa cytoplasmic region. The ECD consists of six leucine-rich repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain, tandemly aligned in that order (1, 2). In addition, there are five potential sites for N-linked glycosylation. SALM4 and -5 lack a C-terminal intracellular PDZ binding domain, which is conserved among SALMs 1‑3. Mature human SALM4 shares 96% aa sequence identity with mature mouse SALM4. Northern blot analysis showed that in mice, SALM4 is strongly expressed in the adult brain and is also present in the adult gastrointestinal tract and kidneys (1). It is distributed throughout the neuron, including the growth cone (3). In the developing mouse embryo, a temporal expression profile blot revealed a general increment of expression around E10.5, with weak expression detected before E10.5 (1). SALM4, like the other SALMs, promotes neurite outgrowth (3). Specifically, the SALMs modify total outgrowth and neurite branching (3).
Morimura, N. et al. (2006) Gene 380:72.
Wang, C.-Y. et al. (2006) J. Neurosci. 26:2174.
Wang, P.Y. et al. (2008) Mol. Cell. Neurosci. 39:83.
Leucine-rich Repeat and Fibronectin Type III Domain Containing Protein 3
Entrez Gene IDs:
79414 (Human); 233067 (Mouse); 308495 (Rat)
fibronectin type III, immunoglobulin and leucine rich repeat domains 1; FIGLER1; leucine rich repeat and fibronectin type III domain containing 3; leucine-rich repeat and fibronectin type-III domain-containing protein 3; LRFN3; MGC2656; SALM4; synaptic adhesion-like molecule 4; UNQ5865/PRO34192
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