Human Serpin E2/PN1 Antibody

Catalog # Availability Size / Price Qty
AF2980
AF2980-SP
Product Details
Citations (2)
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Human Serpin E2/PN1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Serpin E2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse Serpin E2 is observed and less than 1% cross-reactivity with recombinant human (rh) Serpin E1, rhSerpin I1, rhSerpin I2, and rhSerpin B6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin E2
Met1-Pro397
Accession # NP_001130000
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Serpin E2/PN1 (Catalog # 2980-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin E2/PN1

Serpin E2, also known as protease nexin I or glial-derived nexin (GDN), is a member of the Serpin superfamily of the serine protease inhibitors (1). Serpin E2 is a potent inhibitor of thrombin, plasmin and plasminogen activators (2). It is differentially expressed during neuronal differentiation and is able to transform human embryonic kidney cells into neuron-like cells (3). Its over-expression in mice leads to progressive neuronal and motor dysfunction in these animals (4). It is also over-expressed in the majority of pancreatic carcinoma as well as gastric and colorectal cancer samples whereas it is weakly expressed in all normal pancreas and chronic pancreatitis tissue samples (5). It plays an important role in controlling male fertility because its knockout male mice show a marked impairment in fertility from the onset of sexual maturity and its abnormal expression is found in the semen of men with seminal dysfunction (6). The deduced amino acid sequence of rhSerpin E2 is the same as that in NP_001130000, which predicts Arg329 in its 397 amino acid residues (7). An alternatively splice form predicts Thr-Gly at positions 329 and 330 in its 398 amino acid sequence (8-10).

References
  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Rossignol, P. et al. (2004) J. Biol. Chem. 279:10346.
  3. Lin, H.J. et al. (2005) Int. J. Dev. Neurosci. 23:9.
  4. Meins, M. et al. (2001) J. Neurosci. 21:8830.
  5. Buchholz, M. et al. (2003) Cancer Res. 63:4945.
  6. Murer, V. et al. (2001) Proc. Natl. Acad. Sci. USA 98:3029.
  7. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
  8. Sommer, J. et al. (1987) Biochemistry 26:6407.
  9. Gloor, S. et al. (1986) Cell 47:687.
  10. McGrogan, M. et al. (1988) Bio/Technology 6:172.
Entrez Gene IDs
5270 (Human); 20720 (Mouse)
Alternate Names
GDN; GdNPF; GDNserine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 2; glia-derived nexin; glial-derived neurite promoting factor; Peptidase inhibitor 7; PI-7; PI7DKFZp686A13110; PN-1; PN1nexin; PNI; Protease nexin 1; Protease nexin I; Proteinase Nexin 1; Serpin E2; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 2

Product Datasheets

Citations for Human Serpin E2/PN1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Comparison of two neurotrophic serpins reveals a small fragment with cell survival activity
    Authors: PN Winokur, P Subramania, JL Bullock, V Arocas, SP Becerra
    Mol. Vis., 2017;23(0):372-384.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Matrix metalloproteinase-9 regulates tumor cell invasion through cleavage of protease nexin-1.
    Authors: Xu D, McKee CM, Cao Y
    Cancer Res., 2010;70(17):6988-98.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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