Human SGPL1 Antibody

Catalog # Availability Size / Price Qty
AF5535
AF5535-SP
Detection of Human SGPL1 by Western Blot.
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Product Details
Citations (14)
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Human SGPL1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SGPL1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SGPL1
Lys342-His568
Accession # O95470
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SGPL1 antibody by Western Blot. View Larger

Detection of Human SGPL1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, HepG2 human hepatocellular carcinoma cell line, HT-29 human colon adenocarcinoma cell line, and HT-29 tumor tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SGPL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5535) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SGPL1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western Detection of Human SGPL1 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human SGPL1 by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SGPL1 at approximately 60 kDa (as indicated) using 50 µg/mL of Goat Anti-Human SGPL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5535) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SGPL1

SGPL1 (Sphingosine-1-phosphate lyase; also hSPL) is a 60‑63 kDa member of the SGPL1 subfamily, group II decarboxylase family of enzymes. It is very widely expressed, and particularly concentrated in cells undergoing rapid turnover. SGPL1 is found in the ER, and cleaves phosphorylated sphingoid bases into fatty aldehydes and phosphoethanolamine. This regulates a diversity of cellular processes such as apoptosis and T cell migration. Human SGPL1 is 568 amino acids (aa) in length. It is a type III transmembrane protein (luminal N-terminus with no signal sequence) that contains a 507 aa cytoplasmic domain (aa 62‑568). This domain contains a vitamin B6 binding site (aa 344‑364) that is part of a large catalytic region (aa 116‑506). Over aa 342‑568, human SGPL1 shares 86% aa identity with mouse SGPL1.

Long Name
Sphingosine-1-Phosphate Lyase 1
Entrez Gene IDs
8879 (Human); 20397 (Mouse); 286896 (Rat)
Alternate Names
EC 4.1.2.27; FLJ13811; hSPL; KIAA1252; S1PL; SGPL1; Sphingosine-1-phosphate aldolase; sphingosine-1-phosphate lyase 1; SPL; SPLSPL 1; SP-lyase 1; SP-lyase

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Citations for Human SGPL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction
    Authors: A. Maharaj, J. Williams, T. Bradshaw, T. Güran, D. Braslavsky, J. Casas et al.
    The Journal of Steroid Biochemistry and Molecular Biology
  2. Insights From Long-term Follow-up of a Girl With Adrenal Insufficiency and Sphingosine-1-Phosphate Lyase Deficiency
    Authors: Avinaash Maharaj, Tülay Güran, Federica Buonocore, John C Achermann, Louise Metherell, Rathi Prasad et al.
    Journal of the Endocrine Society
  3. A Sphingosine-1-Phosphate Lyase Mutation Associated With Congenital Nephrotic Syndrome and Multiple Endocrinopathy
    Authors: Avinaash Maharaj, Demetria Theodorou, Indraneel (Indi) Banerjee, Louise A. Metherell, Rathi Prasad, Dean Wallace
    Frontiers in Pediatrics
  4. AAV-SPL 2.0, a Modified Adeno-Associated Virus Gene Therapy Agent for the Treatment of Sphingosine Phosphate Lyase Insufficiency Syndrome
    Authors: Khan, R;Oskouian, B;Lee, JY;Hodgin, JB;Yang, Y;Tassew, G;Saba, JD;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Sphingosine Phosphate Lyase Is Upregulated in Duchenne Muscular Dystrophy, and Its Inhibition Early in Life Attenuates Inflammation and Dystrophy in Mdx Mice
    Authors: AS De la Garz, SA Moore, J Zamora-Pin, EP Hoffman, K Mistry, A Kumar, JB Strober, P Zhao, JH Suh, JD Saba
    International Journal of Molecular Sciences, 2022-07-08;23(14):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Efficacy of AAV9-mediated SGPL1 gene transfer in a mouse model of S1P lyase insufficiency syndrome
    Authors: P Zhao, GB Tassew, JY Lee, B Oskouian, DP Muñoz, JB Hodgin, GL Watson, F Tang, JY Wang, J Luo, Y Yang, SM King, RM Krauss, N Keller, JD Saba
    JCI Insight, 2021-04-22;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Heterogenous Nuclear Ribonucleoprotein H1 Promotes Colorectal Cancer Progression through the Stabilization of mRNA of Sphingosine-1-Phosphate Lyase 1
    Authors: K Takahashi, M Fujiya, H Konishi, Y Murakami, T Iwama, T Sasaki, T Kunogi, A Sakatani, K Ando, N Ueno, S Kashima, K Moriichi, H Tanabe, T Okumura
    Int J Mol Sci, 2020-06-25;21(12):.
    Species: Human
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  8. Disarranged Sphingolipid Metabolism From Sphingosine-1-Phosphate Lyase Deficiency Leads to Congenital Nephrotic Syndrome
    Authors: VA Taylor, HK Stone, MP Schuh, X Zhao, KD Setchell, E Erkan
    Kidney Int Rep, 2019-08-07;4(12):1763-1769.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Nicotinamide phosphoribosyltransferase is a molecular target of potent anticancer agents identified from phenotype-based drug screening
    Authors: D Yamaguchi, T Imaizumi, K Yagi, Y Matsumoto, T Nakashima, A Hirose, N Kashima, Y Nosaka, T Hamada, K Okawa, Y Nishiya, K Kubo
    Sci Rep, 2019-05-23;9(1):7742.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Mutations in sphingosine-1-phosphate lyase cause nephrosis with ichthyosis and adrenal insufficiency
    Authors: S Lovric, S Goncalves, HY Gee, B Oskouian, H Srinivas, WI Choi, S Shril, S Ashraf, W Tan, J Rao, M Airik, D Schapiro, DA Braun, CE Sadowski, E Widmeier, T Jobst-Schw, JM Schmidt, V Girik, G Capitani, JH Suh, N Lachaussée, C Arrondel, J Patat, O Gribouval, M Furlano, O Boyer, A Schmitt, V Vuiblet, S Hashmi, R Wilcken, FP Bernier, AM Innes, JS Parboosing, RE Lamont, JP Midgley, N Wright, J Majewski, M Zenker, F Schaefer, N Kuss, J Greil, T Giese, K Schwarz, V Catheline, D Schanze, I Franke, Y Sznajer, AS Truant, B Adams, J Désir, R Biemann, Y Pei, E Ars, N Lloberas, A Madrid, VR Dharnidhar, AM Connolly, MC Willing, MA Cooper, RP Lifton, M Simons, H Riezman, C Antignac, JD Saba, F Hildebrand
    J. Clin. Invest., 2017-02-06;127(3):912-928.
    Species: Mouse, Rat
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  11. Expression of bioactive lysophospholipids and processing enzymes in the vitreous from patients with proliferative diabetic retinopathy.
    Authors: Abu El-Asrar, Ahmed M, Nawaz, Mohd Imt, Mohammad, Ghulam, Siddiquei, Mohammad, Alam, Kaiser, Mousa, Ahmed, Opdenakker, Ghislain
    Lipids Health Dis, 2014-12-11;13(0):187.
    Species: Human
    Sample Types: Vitreous Humor
    Applications: Western Blot
  12. Sphingosine-1-phosphate (S1P) displays sustained S1P1 receptor agonism and signaling through S1P lyase-dependent receptor recycling.
    Authors: Gatfield J, Monnier L, Studer R, Bolli M, Steiner B, Nayler O
    Cell Signal, 2014-04-02;26(7):1576-88.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  13. Increased expression of sphingosine kinase in the amnion during labor.
    Authors: Erkhembaatar L, Kotani T, Sumigama S, Tsuda H, Mano Y, Hua L, Hasegawa Y, Wang J, Sugiyama C, Nakahara T, Iwase A, Kikkawa F
    Placenta, 2013-02-22;34(4):353-9.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  14. ER Stress-Induced Sphingosine-1-Phosphate Lyase Phosphorylation Potentiates the Mitochondrial Unfolded Protein Response
    Authors: Asli D. Yildirim, Mevlut Citir, Asli E. Dogan, Zehra Veli, Zehra Yildirim, Ozlem Tufanli et al.
    Journal of Lipid Research

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