Human SHANK2 Antibody Summary
Accession # Q9UPX8
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
SHANK2 in Human Brain. SHANK2 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human SHANK2 Monoclonal Antibody (Catalog # MAB7035) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
SHANK2 (SH3 and multiple ANKyrin repeat domain protein 2; also CortBP1 and ProSAP1) is a 180-200 kDa member of the Shank family of proteins. It is expressed in neurons and various epithelial cells, and serves as a multidomain scaffold for cell membrane proteins. For example, in the post-synaptic density of neurons, it provides support for the interaction between glutamate receptors and the actin cytoskeleton. In epithelia, SHANK2 regulates the trafficking and activity of Na/H Exchanger 3. Human SHANK2 is 1470 amino acids (aa) in length. It contains an N-terminal SH3 domain (aa 147-206), a PDZ domain (aa 247-341), a Pro-rich region (aa 509-535), a potential O-linked glycosylation site (aa 948-954) and a C-terminal SAM domain (aa 1407-1470). There are seven potential Ser/Thr phosphorylation sites. Multiple splice forms exist. A 220-240 kDa ankyrin repeat-containing isoform (SHANK2-E) possesses a 391 aa substitution for aa 1-12, and a 165 kDa isoform (potentially known as CortBP1) shows a 28 aa substitution for aa 1-238 coupled with a deletion of aa 383-390. Another potential isoform shows the previously noted 28 aa substitution for aa 1-238 coupled to a 19 aa substitution for aa 472-1470. Over aa 849-1029, human SHANK2 shares 80% aa sequence identity with mouse SHANK2.
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Cell and Tissue Staining Kits
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