Human Siglec-6/CD327 Antibody

Catalog # Availability Size / Price Qty
AF2859
AF2859-SP
Human Siglec-6/CD327 Antibody in Data
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Citations (1)
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Human Siglec-6/CD327 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Siglec‑6/CD327 in direct ELISAs and Western blots. In Western blots, approximately 20% cross‑reactivity with recombinant human (rh) Siglec-3, rhSiglec-5 and rhSiglec-9 is observed, approximately 5% cross‑reactivity with rhSiglec-10 is observed, and less than 1% cross‑reactivity with rhSiglec-2, rhSiglec-7, and rhSiglec-11 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Siglec‑6/CD327
Gln27-Val331
Accession # NP_942142
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Siglec‑6 Fc Chimera (Catalog # 2859-SL)
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Siglec‑6/CD327 Monoclonal Antibody (Catalog # MAB28591).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Siglec-6/CD327 DuoSet ELISA Kit (Catalog # DY2859-05) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human Siglec‑6/CD327 ELISA Standard Curve. Recombinant Human Siglec-6/CD327 protein was serially diluted 2-fold and captured by Mouse Anti-Human Siglec-6/CD327 Monoclonal Antibody (Catalog # MAB28591) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Siglec-6/CD327 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2859) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-6/CD327

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins that belong to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1‑4). Eleven human Siglecs (Siglec-1 through 11) have been cloned and characterized. Within these eleven, there are at least two groups, one of which is termed the CD33-related group. CD33-related Siglecs include CD33/Siglec-3 and Siglec-5 through 11 (1, 3). To date, no Siglec has been shown to recognize any cell surface ligand other than sialic acid. This suggests that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. The cDNA of human Siglec-6 (also known as OB-BP1 and CD33L), encodes a putative 442 amino acid (aa) protein that contains a 15 aa signal peptide, a 321 aa extracellular region, a 21 aa transmembrane region (TM), and an 85 aa cytoplasmic tail (5, 6). The extracellular region contains one N-terminal V-type Ig-like domain followed by two Ig-like C2-type domains. The cytoplasmic domain has one immunoreceptor tyrosine-based inhibition motif (ITIM). At least three additional isoforms exist, all of which encode an additional 11 aa’s at the N-terminus, likely due to the utilization of an alternate start site. Two of the three isoforms also show splicing. One isoform shows a 16 aa in-frame deletion in the second C2-like domain, while the other shows a deletion of the TM and cytoplasmic region, thus potentially generating a soluble form (6‑9). Siglec-6 is found on B cells and in placenta, and would seem to have a restricted specificity for the sialyl Tn antigen (6, 10).

References
  1. Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83.
  2. Crocker, P.R. and A. Varki (2001) Immunology 103:137.
  3. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.
  4. Powell, L.D. and A. Varki (1995) J. Biol. Chem. 270:14243.
  5. Takei, Y. et al. (1997) Cytogenet. Cell Genet. 78:295.
  6. Patel, N. et al. (1999) J. Biol. Chem. 274:22729.
  7. GenBank Accession # NP_942143.
  8. GenBank Accession # NP_942142.
  9. GenBank Accession # NP_001236.
  10. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
Long Name
Sialic Acid Binding Ig-like Lectin 6
Entrez Gene IDs
946 (Human)
Alternate Names
CD327 antigen; CD327; CD33 antigen-like 1; CD33L1; CD33L1OBBP1CD327; CD33L2; CD33LCDW327; CDw327; OB-BP1; Obesity-binding protein 1; sialic acid binding Ig-like lectin 6; sialic acid-binding Ig-like lectin 6; Siglec6; Siglec-6

Product Datasheets

Citation for Human Siglec-6/CD327 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Glycodelin-A protein interacts with Siglec-6 protein to suppress trophoblast invasiveness by down-regulating extracellular signal-regulated kinase (ERK)/c-Jun signaling pathway.
    Authors: Lam KK, Chiu PC, Lee CL
    J. Biol. Chem., 2011;286(43):37118-27.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot

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