Detection of Human SOX5 by Western Blot. Western blot shows lysates of human lung tissue and exponentially growing LNCaP human prostate cancer cell line whole cell extract. PVDF membrane was probed with 1 µg/mL of Human SOX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5286) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOX5 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
SOX5 (SRY-related HMG box) is a 75‑80 kDa Group D member of the SOX family of DNA-binding proteins. It is expressed in fetal chondrocytes and skeletal muscle, with a short form appearing in spermatids. SOX5 form homodimers and heterodimerizes with SOX6. Human SOX5 is 763 amino acids (aa) in length and contains two coiled-coil regions (aa 193‑274 and 448‑515), plus one Q/glutamine (aa 234‑272) and one HMG box (aa 556‑624). HMG domains participate in DNA-binding and protein‑protein interaction. There are two alternate start sites at Met14 and Met417 (which creates a short/testis form), plus one variant with a three aa substitution for aa 389‑763, another variant with a two aa substitution for aa 1‑388, and a third variant with a one aa substitution for aa 534‑763. Over aa 417‑763, human SOX5 is 97% aa identical to mouse SOX5.
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