Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Sp3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Sp3
Asp53-Ser309
Accession # Q02447

Specificity

Detects human Sp3 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Sp3 Antibody

Detection of Human Sp3 antibody by Western Blot.

Detection of Human Sp3 by Western Blot.

Western blot shows nuclear extracts of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and, Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Sp3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4256) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Sp3 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Sp3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, and, Daudi human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Sp3

Sp3 is a member of the Sp1 family of C2H2-zinc-finger transcription factors. Sp3 binds to GT and GC boxes in promoters and can either stimulate or repress transcription. Knock out mice indicate that Sp3 is important particularly in erythroid and myeloid cell lineages.

Long Name

Sp3 Transcription Factor

Alternate Names

SPR-2

Entrez Gene IDs

6670 (Human); 20687 (Mouse); 367846 (Rat)

Gene Symbol

SP3

UniProt

Q02447

Additional Sp3 Products

Product Documents for Human Sp3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Sp3 Antibody

For research use only

Customer Reviews for Human Sp3 Antibody

There are currently no reviews for this product. Be the first to review Human Sp3 Antibody and earn rewards!

Have you used Human Sp3 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies