Human SR-BI Antibody

(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human SR-BI in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 947007
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human embryonic kidney cell line HEK293-derived human SR-BI
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunohistochemistry
    5-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human SR-BI by Western Blot. Western blot shows lysates of human liver tissue and human adrenal gland tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SR-BI Monoclonal Antibody (Catalog # MAB8114) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SR-BI at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
SR-BI in Human Liver. SR-BI was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human SR-BI Monoclonal Antibody (Catalog # MAB8114) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SR-BI

Scavenger Receptor, class B, member 1 (SR-B1), gene name SCARB1, is also known as CD36L1 (CD36-like 1) or CLA-1 (CD36 and LIMPII analogous 1) (1-5). SR-B1 is a transmembrane glycoprotein found on macrophages, liver cells and other steroidogenic cells as a lipoprotein receptor. The 552 amino acid (aa) human SR-B1 contains a central extracellular domain (ECD), flanked by N- and C-terminal transmembrane domains. Human splice variants differ at the N-terminal cytoplasmic and transmembrane domains (SR-BIII, 474 aa), the N-terminal end of the ECD (SR-BII, 409 aa), or the C-terminal cytoplasmic domain (isoform 3, 552 aa) (2). The human SR-B1 ECD shares 80%, 80%, 89%, 86% and 84% aa sequence identity with mouse, rat, porcine, rabbit, and bovine SR-B1, respectively. SR-B1 functions in reverse cholesterol transport (RCT), which is thought to be anti-atherogenic by facilitating transport of cholesteryl esters from macrophages back to the liver for degradation (3). In rodent hepatocytes, SR-B1 is the main receptor mediating RCT, while human hepatocytes also express a second mediator, CETP (cholesteryl ester transfer protein) (3-5). The importance of SR-B1 in humans is shown by human SR-B1 genetic variants that alter lipid metabolism (3-7). For example, the P297S polymorphism lowers uptake of high-density lipoprotein (HDL) cholesterol in the liver and increases plasma HDL cholesterol (3-5). On endothelial cells, signaling through SR-B1 activates nitric oxide production, which attenuates monocyte adhesion (6). On adrenocortical cells, SR-B1 mediates uptake of cholesteryl esters from HDL for the synthesis of glucocorticoid hormones such as cortisol (3-5). On platelets, HDL binding to surface SR-B1 inhibits aggregation and increases platelet survival time (3-5). On human ovarian granulosa cells, deficiency of SR-B1 correlates with low fertility (3). SR-B1 and its SR-BII isoform also bind bacterial lipopolysaccharides, facilitating uptake of various bacteria by cells such as peritoneal macrophages (8, 9). This uptake enhances inflammatory responses which, unless properly controlled, can result in sepsis (9-11).

  • References:
    1. Calvo, D. and M. A. Vega (1993) J. Biol. Chem. 268:18929.
    2. Swiss-Prot accession Q8WTV0
    3. Chadwick, A.C. and D. Sahoo (2013) Curr. Opin. Endocrinol. Diabetes Obes. 20:124.
    4. Hoekstra, M. et al. (2012) Curr. Opin. Lipidol. 23:127.
    5. Vergeer, M. et al. (2011) N. Engl. J. Med. 364:136.
    6. Guo, L. et al. (2011) J. Lipid Res. 52:2272.
    7. Saddar, S. et al. (2012) Circ. Res. 112:140.
    8. Vishnyakova, T.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16888.
    9. Baranova, I.N. et al. (2012) J. Immunol. 188:1371.
    10. Leelahavanichkul, A. et al. (2012) J. Immunol. 188:2749.
    11. Guo, L. et al. (2009) J. Biol. Chem. 284:19826.
  • Long Name:
    Scavenger Receptor Class B, Member I
  • Entrez Gene IDs:
    949 (Human); 20778 (Mouse); 25073 (Rat)
  • Alternate Names:
    CD36 and LIMPII analogous 1; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1; CD36 antigen; CD36L1; CD36L1scavenger receptor class B type 1; CLA1 CD36 antigen-like 1; CLA-1 HDLQTL6; CLA1; Collagen type I receptor, thrombospondin receptor-like 1; HDLQTL6; MGC138242; SCARB1; scavenger receptor class B type III; scavenger receptor class B, member 1; SRB1 scavenger receptor class B member 1; SR-B1; SRBI; SR-BI
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Average Rating: 4 (Based on 1 review)

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Summary

ApplicationFlow Cytometry
Sample TestedRecombinant protein,Liver cells

Other Experimental Details

Other Experimental Detailsalso FACS, and functional assay

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