Human SREC-I/SCARF1 Antibody

Catalog # Availability Size / Price Qty
AF2409
AF2409-SP
Detection of SREC‑I/SR‑F1 in HUVEC Human Cells by Flow Cytometry.
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Product Details
Citations (4)
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Reviews (1)

Human SREC-I/SCARF1 Antibody Summary

Species Reactivity
Human
Specificity
Detects SREC‑I/SCARF1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) SREC-II is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SREC‑I/SCARF1
Ser20-Thr421
Accession # Q14162
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human SREC-I/SR-F1 Fc Chimera (Catalog # 2409-SR)
Flow Cytometry
2.5 µg/106 cells
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.03-0.1 µg/mL of this antibody will block 50% of the binding of 20 ng/mL of Recombinant Human SREC-I Fc Chimera (Catalog # 2409-SR) to immobilized human acetylated low-density lipoproteins (AcLDL) coated at 2 µg/mL (100 µL/well). At 0.5 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of SREC-I/SR-F1 antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of SREC‑I/SR‑F1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human SREC-I/SR-F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2409, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SREC-I/SCARF1

The scavenger receptor (SR) family comprises a group of functionally defined membrane receptors that share a common ability to bind and internalize modified forms of low density lipoproteins (LDL) such as acetylated LDL (AcLDL) and oxidized LDL (OxLDL) (1‑3). Family members are classified alphabetically. They play important roles in lipid metabolism, in host defence and in the regulation of acquired immunity (2, 4). Scavenger receptor expressed by endothelial cells-I (SREC-I) and SREC-II are two proteins that belong to the F type scavenger receptor group (SR-F1 and SR-F2). The full length cDNA of human SREC-I encodes an 830 amino acid (aa) type I transmembrane protein which contains a 19 aa signal peptide, a 402 aa extracellular region, a 21 aa transmembrane segment, and a 388 aa long cytoplasmic domain. The extracellular region contains ten EGF-like repeats (five of which fit the exact consensus sequence for an EGF-like domain) while the cytoplasmic domain is rich in serine and proline in the N-terminal half, and glycine in the C-terminal segment (5, 6). In addition to the full length form, four SREC-I isoforms exist. Two show insertions of a stop codon in EGF-like domain #8, resulting in mature soluble forms of 323 aa and 318 aa, respectively. A third isoform deletes part of domain #8 plus domains #9 and #10; it continues in-frame to generate a mature transmembrane protein of 725 aa. The last isoform shows only cytoplasmic splicing, with 72 aa substituted for the last 332 aa of the full length form. All three transmembrane forms bind acetylated LDL (6). Native SREC-I is approximately 150 kDa in size, and expressed by endothelial cells, macrophages and fetal neurons (7, 8). In the extracellular region human SREC-I is 76% aa identical to mouse SREC-I. The extracellular regions of human SREC-I and II are 53% aa identical.

References
  1. Horiuchi, S. et al. (2003) Amino Acids 25:283.
  2. Greaves, D.R. and S. Gordon (2005) J. Lipid Res. 46:11.
  3. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
  4. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
  5. Adachi, H. et al. (1997) J. Biol. Chem. 272:31217.
  6. Adachi, H. and M. Tsujimoto (2002) J. Biol. Chem. 277:24014.
  7. Shibata, M. et al. (2004) J. Biol. Chem. 279:40084.
  8. Tanura, Y. et al. (2004) J. Biol. Chem. 279:30938.
Long Name
Scavenger Receptor Expressed by Endothelial Cells 1
Entrez Gene IDs
8578 (Human)
Alternate Names
Acetyl LDL receptor; KIAA0149endothelial cells scavenger receptor; MGC47738; SCARF1; scavenger receptor class F, member 1; Scavenger receptor expressed by endothelial cells 1; SREC1; SRECI; SREC-I; SRECscavenger receptor expressed by endothelial cells; SR-F1

Product Datasheets

Citations for Human SREC-I/SCARF1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. A nasal epithelial receptor for Staphylococcus aureus WTA governs adhesion to epithelial cells and modulates nasal colonization.
    Authors: Baur S, Rautenberg M, Faulstich M, Grau T, Severin Y, Unger C, Hoffmann W, Rudel T, Autenrieth I, Weidenmaier C
    PLoS Pathog, 2014;10(5):e1004089.
    Species: Cotton Rat
    Sample Types: Whole Cells
    Applications: ICC
  2. Scavenger receptors in human airway epithelial cells: role in response to double-stranded RNA.
    Authors: Dieudonne A, Torres D, Blanchard S, Taront S, Jeannin P, Delneste Y, Pichavant M, Trottein F, Gosset P
    PLoS ONE, 2012;7(8):e41952.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Oligo-guanosine nucleotide induces neuropilin-1 internalization in endothelial cells and inhibits angiogenesis.
    Authors: Narazaki M, Segarra M, Hou X, Tanaka T, Li X, Tosato G
    Blood, 2010;116(16):3099-107.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Sulfated polysaccharides identified as inducers of neuropilin-1 internalization and functional inhibition of VEGF165 and semaphorin3A.
    Authors: Narazaki M, Segarra M, Tosato G
    Blood, 2008;111(8):4126-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human SREC-I/SCARF1 Antibody
By Anonymous on 01/20/2017
Application: Flow Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human