Human SRPK2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human SRPK2 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human SRPK2
    Asn313-Ala466
    Accession # P78362
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human SRPK2 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Human SRPK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5530) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SRPK2 at approximately 115 to 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SRPK2

SRPK2 (Ser/Arg-rich protein specific kinase 2) is a cytoplasmic, 115‑120 kDa member of the CMGC Ser/Thr protein kinase family of enzymes. It is expressed in many experimental cell lines and serves to selectively phosphorylate Ser on RS domain-containing proteins. This phosphorylation activates RS domain-containing non-snRNP proteins in the spliceosome complex, and initiates mRNA splicing. Human SRPK2 is 688 amino acids (aa) in length. It contains a Pro-rich region (aa 21‑45) that may interact with WW domain-containing proteins, and a split kinase domain (aa 79‑226 and 524‑646). Phosphorylation on S52 and S588 regulates activity. There are at least two splice variants. One shows a 24 aa substitution for aa 1‑13, while a second shows a nine aa substitution for aa 538‑688.

  • Long Name:
    SFRS Protein Kinase 2
  • Entrez Gene IDs:
    6733 (Human); 20817 (Mouse); 296753 (Rat)
  • Alternate Names:
    EC 2.7.11.1; FLJ36101; H_RG152G17.1a; H_RG152G17.1b; serine kinase SRPK2; Serine/arginine-rich protein-specific kinase 2; serine/arginine-rich splicing factor kinase 2; serine/threonine-protein kinase SRPK2; SFRS protein kinase 2SFRSK2; SFRSK2; SR protein kinase 2; SRPK2; SR-protein-specific kinase 2; SRSF protein kinase 2; WUGSC:H_RG152G17.1a
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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