Human STAT5b APC-conjugated Antibody

Name: Human STAT5b APC-conjugated Antibody
Brand: R&D Systems
SKU: IC1584A

Catalog #: IC1584A
1 Citation Datasheet / COA / SDS

Discontinued Product

IC1584A has been discontinued.

View all STAT5b products.

Detection of STAT5b in Jurkat Human Cell Line by Flow Cytometry.

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All STAT5b Antibodies

Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human STAT5b APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human STAT5b in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 389215

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human STAT5b synthetic peptide
MDSQWIPHAQS
Accession # NP_036580

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of STAT5b antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

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Detection of STAT5b in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human STAT5b APC-conjugated Monoclonal Antibody (Catalog # IC1584A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: STAT5b

Signal Transduction and Activator of Transcription 5B (STAT5b) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL-22, IL-6, IFN-alpha ). Upon cytokine activation, STAT5b is phosphorylated and translocates to the nucleus where it binds to the DNA consensus site TTCN₃GAA and activates transcription of specific genes. STAT5b shares 93% amino acid identity with STAT5a. STAT5b is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival.

Long Name

Signal Transducer and Activator of Transcription 5b

Entrez Gene IDs

6777 (Human); 20851 (Mouse)

Alternate Names

signal transducer and activator of transcription 5B; STAT5; STAT5b; transcription factor STAT5B

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human STAT5b APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

TGF-beta induces the differentiation of human CXCL13-producing CD4(+) T cells.
Authors: Kobayashi S, Watanabe T, Suzuki R, Furu M, Ito H, Ito J, Matsuda S, Yoshitomi H
Eur J Immunol, 2015-11-24;46(2):360-71.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry