Human STAT5b APC-conjugated Antibody

IC1584A has been discontinued. View all STAT5b products.
(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human STAT5b in Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 389215
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human STAT5b synthetic peptide
    MDSQWIPHAQS
    Accession # NP_036580
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry

Detection of STAT5b in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human STAT5b APC‑conjugated Monoclonal Antibody (Catalog # IC1584A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: STAT5b
Signal Transduction and Activator of Transcription 5B (STAT5b) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL-22, IL-6, IFN-alpha ). Upon cytokine activation, STAT5b is phosphorylated and translocates to the nucleus where it binds to the DNA consensus site TTCN3GAA and activates transcription of specific genes. STAT5b shares 93% amino acid identity with STAT5a. STAT5b is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival.
  • Long Name:
    Signal Transducer and Activator of Transcription 5b
  • Entrez Gene IDs:
    6777 (Human); 20851 (Mouse)
  • Alternate Names:
    signal transducer and activator of transcription 5B; STAT5; STAT5b; transcription factor STAT5B
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. TGF-beta induces the differentiation of human CXCL13-producing CD4(+) T cells.
    Authors: Kobayashi S, Watanabe T, Suzuki R, Furu M, Ito H, Ito J, Matsuda S, Yoshitomi H
    Eur J Immunol, 2015;46(2):360-71.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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