Human SUSD2 Antibody

Catalog # Availability Size / Price Qty
MAB90561
MAB90561-SP
Detection of SUSD2  in HEK293 Human Cell Line Transfected with Human SUSD2 and eGFP by Flow  Cytometry.
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Human SUSD2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SUSD2 in direct ELISAs. Stains human SUSD2 transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2A Clone # 944827
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived human SUSD2
Met1-Ala785
Accession # Q9UGT4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of SUSD2  in HEK293 Human Cell Line Transfected with Human SUSD2 and eGFP by Flow  Cytometry. View Larger

Detection of SUSD2 in HEK293 Human Cell Line Transfected with Human SUSD2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human SUSD2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human SUSD2 Monoclonal Antibody (Catalog # MAB90561) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SUSD2

Sushi domain containing 2, or SUSD2, is a type I transmembrane protein of 822 amino acids containing functional domains inherent to adhesion molecules. SUSD2 has been described as a novel marker of human endometrial mesenchymal stem-like cells and it has been used for their prospective isolation. As a transmembrane receptor, SUSD2 has been proposed to interact with Galectin-1 and to be the receptor for C10ORF99, a novel potential cytokine suggested to inhibit colon cancer cell growth through inducing G1 arrest. There is  evidence that SUSD2 may play a role in breast tumorigenesis.

Long Name
Sushi Domain Containing 2
Entrez Gene IDs
56241 (Human); 71733 (Mouse); 294335 (Rat)
Alternate Names
BK65A6.2; FLJ22778; SUSD2; Sushi domain (SCR repeat) containing; sushi domain containing 2; sushi domain-containing protein 2

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Mouse IgG2A Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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