Detection of Human Syntabulin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Syntabulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5544) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Syntabulin at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Syntabulin (also Golgi-localized synaphilin-related protein) is a 70-75 kDa molecule that belongs to the syntaxin-binding and kinesin-1 family of motor adaptor proteins. It is highly spliced, with various forms associated with different tissues. In neurons, it anchors syntaxin-1 to KIF5B, creating a complex by which synaptic components can be transported to synaptic terminals. Human Syntabulin is a type II transmembrane (TM) protein 663 amino acids (aa) in length. It contains one coiled-coil region (aa 271‑353) and a TM domain between aa 606‑626. Alternate splice forms show alternate start sites at Met120 and Met207, a five aa substitution for aa 1‑8, a 16 aa insertion after Lys8, and an eight aa substitution for aa 1‑76. Over aa 486‑653, human Syntabulin shares 83% aa identity with mouse Syntabulin.
Entrez Gene IDs:
55638 (Human); 319613 (Mouse); 500865 (Rat)
Golgi-Localized Protein; Golgi-Localized Syntaphilin-Related Protein; GOLSYN A Protein; GOLSYN B Protein; GOLSYN C Protein; GOLSYN; Implicated In Syntaxin Trafficking In Neurons; KIAA1472; Microtubule-Associated Protein; OCSYN; SNPHL; SYBU; Syntabulin (Syntaxin-Interacting); Syntabulin; syntaphilin-like; Syntaxin-1-Binding Protein
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