Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Syntaxin-BP3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Syntaxin‑BP3
Thr84-Thr231
Accession # O00186

Specificity

Detects human Syntaxin‑BP3 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) STXBP1 and rhSTXBP2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Syntaxin‑BP3 Antibody

Detection of Human Syntaxin-BP3 antibody by Western Blot.

Detection of Human Syntaxin-BP3 by Western Blot.

Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Syntaxin-BP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5659) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Syntaxin-BP3 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Syntaxin‑BP3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Nalm‑6 human Pre‑B acute lymphocytic leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Syntaxin-BP3

Syntaxin‑BP3 (Syntaxin-binding protein 3; also PSP, Munc18-C and -3; and Unc-18C) is a 66‑70 kDa member of the SNARE-interacting Sec1/Munc18 family of proteins. It is expressed in cells that exhibit granule exocytosis, such as neutrophils, mast cells, platelets and endothelial cells. Functionally, it likely participates in the consolidation and secretion of secondary and tertiary granules. Human Syntaxin‑BP3 is 592 amino acids (aa) in length. It contains one SEC1 domain (aa 16‑592). Phosphorylation at Ser129 may stimulate granule release. There are two potential splice variants. One shows a 12 aa substitution for aa 31‑269, while a second shows a one aa Gln insertion after Lys270. Over aa 84‑231, human Syntaxin‑BP3 shares 90% aa identity with mouse Syntaxin‑BP3.

Long Name

Syntaxin-binding Protein 3

Alternate Names

Munc18c, STXBP3, SyntaxinBP3, Unc-18C

Entrez Gene IDs

6814 (Human); 20912 (Mouse); 114095 (Rat)

Gene Symbol

STXBP3

UniProt

O00186 (Human)

Additional Syntaxin-BP3 Products

Product Documents for Human Syntaxin‑BP3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Syntaxin‑BP3 Antibody

For research use only

Citations for Human Syntaxin‑BP3 Antibody

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