Detection of Human Syntaxin-BP3 by Western Blot. |
Western blot shows lysates of Nalm‑6 human Pre‑B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Syntaxin-BP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5659) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Syntaxin-BP3 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Syntaxin‑BP3 (Syntaxin-binding protein 3; also PSP, Munc18-C and -3; and Unc-18C) is a 66‑70 kDa member of the SNARE-interacting Sec1/Munc18 family of proteins. It is expressed in cells that exhibit granule exocytosis, such as neutrophils, mast cells, platelets and endothelial cells. Functionally, it likely participates in the consolidation and secretion of secondary and tertiary granules. Human Syntaxin‑BP3 is 592 amino acids (aa) in length. It contains one SEC1 domain (aa 16‑592). Phosphorylation at Ser129 may stimulate granule release. There are two potential splice variants. One shows a 12 aa substitution for aa 31‑269, while a second shows a one aa Gln insertion after Lys270. Over aa 84‑231, human Syntaxin‑BP3 shares 90% aa identity with mouse Syntaxin‑BP3.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided