T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62 kDa member of the T-box family of transcription factors and the Tbr1 subfamily. Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1-cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN gamma, and initiates Th1 lineage development from naïve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1091005
Loading...
Product Specifications
Immunogen
E. coli-derived recombinant human T-bet
Glu326-Asp535
Accession # Q9UL17
Glu326-Asp535
Accession # Q9UL17
Specificity
Detects recombinant human T-bet protein in Direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human T‑bet/TBX21 Antibody
Detection of Human T‑bet/TBX21 by Western Blot.
Western Blot shows lysates of Jurkat human acute T cell leukemia cell line, SUP‑T1 human T cell lymphoblastic lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/ml of Mouse Anti-Human T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB11637) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for T‑bet/TBX21 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Human T‑bet/TBX21 by Simple WesternTM.
Simple Western shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.5 mg/ml. A specific band was detected for T‑bet/TBX21 at approximately 54 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB11637). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human T‑bet/TBX21 Antibody
Application
Recommended Usage
Simple Western
20 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line
Sample: Daudi human Burkitt's lymphoma cell line
Western Blot
0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, SUP-T1 human T cell lymphoblastic lymphoma cell line and Daudi human Burkitt's lymphoma cell line
Sample: Jurkat human acute T cell leukemia cell line, SUP-T1 human T cell lymphoblastic lymphoma cell line and Daudi human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: T-bet/TBX21
Long Name
T-box transcription factor TBX21
Alternate Names
T-PET, Tbet, TBLYM, TBX21
Gene Symbol
TBX21
UniProt
Additional T-bet/TBX21 Products
Product Documents for Human T‑bet/TBX21 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human T‑bet/TBX21 Antibody
For research use only
Customer Reviews for Human T‑bet/TBX21 Antibody
There are currently no reviews for this product. Be the first to review Human T‑bet/TBX21 Antibody and earn rewards!
Have you used Human T‑bet/TBX21 Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...
Associated Pathways
Th1 Differentiation Pathway
