Human T-bet/TBX21 Antibody

Catalog #: MAB11637 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11637-100
MAB11637-SP

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Detection of Human T‑bet/TBX21 by Western Blot.

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All T-bet/TBX21 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human T-bet/TBX21 Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human T-bet protein in Direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1091005

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human T-bet
Glu326-Asp535
Accession # Q9UL17

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

Jurkat human acute T cell leukemia cell line, SUP-T1 human T cell lymphoblastic lymphoma cell line and Daudi human Burkitt's lymphoma cell line

Simple Western

20 µg/mL

Daudi human Burkitt's lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human T‑bet/TBX21 by Western Blot. Western Blot shows lysates of Jurkat human acute T cell leukemia cell line, SUP‑T1 human T cell lymphoblastic lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/ml of Mouse Anti-Human T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB11637) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for T‑bet/TBX21 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western

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Detection of Human T‑bet/TBX21 by Simple Western^TM. Simple Western shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.5 mg/ml. A specific band was detected for T‑bet/TBX21 at approximately 54 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB11637). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: T-bet/TBX21

T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62 kDa member of the T-box family of transcription factors and the Tbr1 subfamily. Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1-cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN gamma, and initiates Th1 lineage development from naïve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.

Long Name

T-box transcription factor TBX21

Entrez Gene IDs

30009 (Human); 57765 (Mouse); 303496 (Rat)

Alternate Names

Tbet; T-bet; TBETT-PET; TBLYM; TBLYMT-cell-specific T-box transcription factor T-bet; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor TBX21; TBX21; T-PET; Transcription factor TBLYM