Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TACC3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TACC3
Glu689-Ile838
Accession # Q9Y6A5

Specificity

Detects human TACC3 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TACC3 Antibody

Detection of Human TACC3 antibody by Western Blot.

Detection of Human TACC3 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TACC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5720) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TACC3 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human TACC3 antibody by Simple WesternTM.

Detection of Human TACC3 by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for TACC3 at approximately 149 kDa (as indicated) using 10 µg/mL of Goat Anti-Human TACC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5720) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human TACC3 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TACC3

Transforming acidic coiled-coil containing protein 3 (TACC3; also know as ERIC-1) is a 92‑140 kDa member of the TACC family of transcriptional regulatory proteins. It is expressed in a variety of cell types, including Sertoli cells, endothelial cells, erythroid progenitors, and thyroid epithelium. When phosphorylated by Aurora A on Ser558, it stabilizes the mitotic spindle. It also interacts with nuclear histone acetyltransferases, promoting their positive effect on transcription. Human TACC3 is 838 amino acids (aa) in length and contains one poly-Serine region (aa 155‑160) and a TACC coiled-coil domain (aa 637‑837). There is one potential splice variant that shows a Lys substitution for aa 102‑462. Over amino acids 689‑838, human TACC3 shares 79% aa identity with mouse TACC3.

Long Name

Transforming Acidic Coiled-coil-containing Protein 3

Alternate Names

ERIC-1

Entrez Gene IDs

10460 (Human); 21335 (Mouse); 360962 (Rat)

Gene Symbol

TACC3

UniProt

Q9Y6A5 (Human)

Additional TACC3 Products

Product Documents for Human TACC3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TACC3 Antibody

For research use only

Citations for Human TACC3 Antibody

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