Human Talin2 Antibody

Catalog # Availability Size / Price Qty
AF4738
AF4738-SP
Detection of Human Talin2 by Western Blot.
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Human Talin2 Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human Talin2 in Western blots. In Western blots, no cross-reactivity to recombinant human Talin1 was detected.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Talin2
Ala977-Arg1183
Accession # Q9Y4G6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Talin2 antibody by Western Blot. View Larger

Detection of Human Talin2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Talin2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4738) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Talin2 at approximately 240 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Talin2

Talin2 (talus; Latin for ankle, the shape of a focal adhesion plaque complex) is a 240 kDa cytoplasmic focal adhesion molecule. It likely forms dimers that link integrins to the actin cytoskeleton. Human Talin2 is 2542 amino acids (aa) in length. It contains one globular N‑terminal FERM domain that binds integrin cytodomains (aa 88‑406), followed by an Ala-rich region (aa 861‑1193) and a C‑terminal actin-binding domain (aa 2321‑2530). Human Talin2 has three potential variants. Two show alternate start sites at Met19 and Met1153, while a third has an alternate start site at Met1608 accompanied by a deletion of aa 1748‑1790. Over aa’s 977‑1183, human Talin2 shares 99% and 97% aa identity with canine and mouse Talin2, respectively.

Entrez Gene IDs
83660 (Human); 70549 (Mouse); 315776 (Rat)
Alternate Names
DKFZp451B1011; DKFZp686I0976; DKFZp686K0979; ILWEQ; KIAA0320; Talin2; talin-2; TLN 2; TLN2

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