Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 668710
View all TBX20 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TBX20
Met1-Leu116
Accession # Q9UMR3

Specificity

Detects human TBX20 in ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human TBX20 Antibody

TBX20 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

TBX20 in BG01V Human Embryonic Stem Cells.

TBX20 was detected in immersion fixed BG01V human embryonic stem cells differentiated to cardiac cells using Mouse Anti-Human TBX20 Monoclonal Antibody (Catalog # MAB8124) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human TBX20 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells differentiated to cardiac cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TBX20

TBX20 (T-box transcription factor 20) is a transcriptional activator and repressor that is essential for cardiac development and to adult cardiomyocyte function. Mutation in humans or mice can result in congenital heart disease, including septation, chamber, and valvulogenesis defects and dilated cardiomyopathy. These include atrial septal defect 4 (ASD4). The 447 amino acid (aa) human TBX20 contains a T-box DNA binding domain at aa 109-228. Between aa 1-116, human TBX20 shares 96% aa sequence identity with mouse TBX20. One isoform that is truncated at aa 297 has been described.

Long Name

T-box Transcription Factor 20

Alternate Names

ASD4

Entrez Gene IDs

57057 (Human); 57246 (Mouse)

Gene Symbol

TBX20

UniProt

Q9UMR3

Additional TBX20 Products

Product Documents for Human TBX20 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TBX20 Antibody

For research use only

Citations for Human TBX20 Antibody

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