Human TBX20 Antibody

Catalog # Availability Size / Price Qty
MAB8124
MAB8124-SP
Human TBX20 Antibody in Immunocytochemistry
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Human TBX20 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TBX20 in ELISA.
Source
Monoclonal Mouse IgG2A Clone # 668710
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TBX20
Met1-Leu116
Accession # Q9UMR3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry TBX20 in BG01V Human Embryonic Stem Cells. View Larger

TBX20 in BG01V Human Embryonic Stem Cells. TBX20 was detected in immersion fixed BG01V human embryonic stem cells differentiated to cardiac cells using Mouse Anti-Human TBX20 Monoclonal Antibody (Catalog # MAB8124) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TBX20

TBX20 (T-box transcription factor 20) is a transcriptional activator and repressor that is essential for cardiac development and to adult cardiomyocyte function. Mutation in humans or mice can result in congenital heart disease, including septation, chamber, and valvulogenesis defects and dilated cardiomyopathy. These include atrial septal defect 4 (ASD4). The 447 amino acid (aa) human TBX20 contains a T-box DNA binding domain at aa 109-228. Between aa 1-116, human TBX20 shares 96% aa sequence identity with mouse TBX20. One isoform that is truncated at aa 297 has been described.

Long Name
T-box Transcription Factor 20
Entrez Gene IDs
57057 (Human); 57246 (Mouse)
Alternate Names
ASD4; T-box 20; T-box protein 20; T-box transcription factor TBX20; TBX20

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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