Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TFCP2L1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TFCP2L1
Met1-Lys180
Accession # Q9NZI6

Specificity

Detects human TFCP2L1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TFCP2L1 Antibody

Detection of Human TFCP2L1 antibody by Western Blot.

Detection of Human TFCP2L1 by Western Blot.

Western blot shows lysates of human placental tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TFCP2L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5726) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TFCP2L1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of TFCP2L1 by Western Blot

Detection of TFCP2L1 by Western Blot

RSK mutations increase the rate of entry into differentiationRSK1*23 and parental cells were withdrawn from 2iLIF and cell lysates prepared between 40 and 48 h. Immunoblotting was performed with indicated antibodies. Ctrl = parental line. Marker gene expression analysis by RT–qPCR in RSK1*23 mutants and parental cells transferred from 2iLIF into N2B27 for indicated times. Mean and SD shown; n = 2.Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29895711), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human TFCP2L1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human placental tissue

Reviewed Applications

Read 2 reviews rated 5 using AF5726 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: TFCP2L1

TFCP2L1 (Transcription factor CP2‑like protein # 1; also LBP‑9) is a 54 kDa (predicted) member of the CP2 subfamily, grh/CP2 family of proteins. It is expressed in placental synctiotrophoblast cells, and regulates the production of CYP11A1/P450scc. P450scc is a cholesterol cleavage enzyme that generates pregnenolone, a necessary intermediate in the formation of progesterone. TFCP2L1 both positively and negatively regulates P450scc synthesis through homodimerization and heterodimerization with LBP‑1b. Human TFCP2L1 is 479 amino acids (aa) in length. It contains a transcriptional repressor region (aa 100‑200) and a dimerization domain (aa 300‑479). There is one splice variant that shows a deletion of aa 400‑464. Human TFCP2L1 is 73% aa identical to human TFCP2, and 93% aa identical to mouse CRTR‑1, a closely related molecule that may not represent the actual mouse ortholog to TFCP2L1.

Long Name

Transcription factor CP2-like 1

Alternate Names

CRTR1, LBP9

Entrez Gene IDs

29842 (Human); 81879 (Mouse); 304741 (Rat)

Gene Symbol

TFCP2L1

UniProt

Q9NZI6

Additional TFCP2L1 Products

Product Documents for Human TFCP2L1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human TFCP2L1 Antibody

For research use only

Citations for Human TFCP2L1 Antibody

Customer Reviews for Human TFCP2L1 Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human TFCP2L1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Human TFCP2L1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human embryonic stem cells (H9 from Wicell)
    Species: Human
    Verified Customer | Posted 05/31/2018
    1:500 concentration used
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: R1 wild type Mouse embryonic Stem Cells
    Species: Mouse
    Verified Customer | Posted 08/04/2016
    Tfcp2l1 in R1 wild type mouse embryonic stem cells
    Human TFCP2L1 Antibody AF5726

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies