Human TFCP2L1 Antibody

Catalog # Availability Size / Price Qty
AF5726
AF5726-SP
Human TFCP2L1 Antibody in Data
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Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (1)

Human TFCP2L1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TFCP2L1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TFCP2L1
Met1-Lys180
Accession # Q9NZI6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human TFCP2L1 antibody by Western Blot. View Larger

Detection of Human TFCP2L1 by Western Blot. Western blot shows lysates of human placental tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TFCP2L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5726) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TFCP2L1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFCP2L1

TFCP2L1 (Transcription factor CP2‑like protein # 1; also LBP‑9) is a 54 kDa (predicted) member of the CP2 subfamily, grh/CP2 family of proteins. It is expressed in placental synctiotrophoblast cells, and regulates the production of CYP11A1/P450scc. P450scc is a cholesterol cleavage enzyme that generates pregnenolone, a necessary intermediate in the formation of progesterone. TFCP2L1 both positively and negatively regulates P450scc synthesis through homodimerization and heterodimerization with LBP‑1b. Human TFCP2L1 is 479 amino acids (aa) in length. It contains a transcriptional repressor region (aa 100‑200) and a dimerization domain (aa 300‑479). There is one splice variant that shows a deletion of aa 400‑464. Human TFCP2L1 is 73% aa identical to human TFCP2, and 93% aa identical to mouse CRTR‑1, a closely related molecule that may not represent the actual mouse ortholog to TFCP2L1.

Long Name
Transcription factor CP2-like 1
Entrez Gene IDs
29842 (Human); 81879 (Mouse); 304741 (Rat)
Alternate Names
CRTR1; CRTR1CRTR-1; LBP9; LBP-9; LBP9CP2-related transcriptional repressor 1; TFCP2L1; transcription factor CP2-like 1; transcription factor CP2-like protein 1; Transcription factor LBP-9

Product Datasheets

Citations for Human TFCP2L1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Critical Roles of Translation Initiation and RNA Uridylation in Endogenous Retroviral Expression and Neural Differentiation in Pluripotent Stem Cells
    Authors: K Takahashi, D Jeong, S Wang, M Narita, X Jin, M Iwasaki, SD Perli, BR Conklin, S Yamanaka
    Cell Rep, 2020;31(9):107715.
    Species: Human
    Sample Types:
    Applications: ICC
  2. Identification of ALPPL2 as a Naive Pluripotent State-Specific Surface Protein Essential for Human Naive Pluripotency Regulation
    Authors: Y Bi, Z Tu, Y Zhang, P Yang, M Guo, X Zhu, C Zhao, J Zhou, H Wang, Y Wang, S Gao
    Cell Rep, 2020;30(11):3917-3931.e5.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency
    Authors: N Bredenkamp, J Yang, J Clarke, GG Stirparo, F von Meyenn, S Dietmann, D Baker, R Drummond, Y Ren, D Li, C Wu, M Rostovskay, S Eminli-Mei, A Smith, G Guo
    Stem Cell Reports, 2019;13(6):1083-1098.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Transcription Factors Drive Tet2-Mediated Enhancer Demethylation to Reprogram Cell Fate
    Authors: JL Sardina, S Collombet, TV Tian, A Gómez, B Di Stefano, C Berenguer, J Brumbaugh, R Stadhouder, C Segura-Mor, M Gut, IG Gut, S Heath, S Aranda, L Di Croce, K Hochedling, D Thieffry, T Graf
    Cell Stem Cell, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunoprecipitation
  5. Honey bee Royalactin unlocks conserved pluripotency pathway in mammals
    Authors: DC Wan, SL Morgan, AL Spencley, N Mariano, EY Chang, G Shankar, Y Luo, TH Li, D Huh, SK Huynh, JM Garcia, CM Dovey, J Lumb, L Liu, KV Brown, A Bermudez, R Luong, H Zeng, VL Mascetti, SJ Pitteri, J Wang, H Tu, M Quarta, V Sebastiano, R Nusse, TA Rando, JE Carette, JF Bazan, KC Wang
    Nat Commun, 2018;9(1):5078.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys
    Authors: Y Kang, Z Ai, K Duan, C Si, Y Wang, Y Zheng, J He, Y Yin, S Zhao, B Niu, X Zhu, L Liu, L Xiang, L Zhang, Y Niu, W Ji, T Li
    Cell Rep, 2018;25(9):2563-2576.e9.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Whole Cells
    Applications: ICC
  7. Trim33 regulates early maturation of mouse embryoid bodies in vitro
    Authors: S Rajderkar, C Panaretos, V Kaartinen
    Biochem Biophys Rep, 2017;12(0):185-192.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. alpha-Intercalated cells defend the urinary system from bacterial infection.
    Authors: Paragas N, Kulkarni R, Werth M, Schmidt-Ott K, Forster C, Deng R, Zhang Q, Singer E, Klose A, Shen T, Francis K, Ray S, Vijayakumar S, Seward S, Bovino M, Xu K, Takabe Y, Amaral F, Mohan S, Wax R, Corbin K, Sanna-Cherchi S, Mori K, Johnson L, Nickolas T, D'Agati V, Lin C, Qiu A, Al-Awqati Q, Ratner A, Barasch J
    J Clin Invest, 2014;124(7):2963-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Human TFCP2L1 Antibody
By Anonymous on 05/31/2018
Application: WB Sample Tested: Human embryonic stem cells (H9 from Wicell) Species: Human

1:500 concentration used