Human Thrombopoietin R/Tpo R Antibody

Catalog # Availability Size / Price Qty
Cell Proliferation Induced by Thrombopoietin/Tpo and Neutralization by Human Thrombopoietin R/Tpo R Antibody.
2 Images
Product Details
Citations (3)
Supplemental Products

Human Thrombopoietin R/Tpo R Antibody Summary

Species Reactivity
Detects human Thrombopoietin R/Tpo R in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human Epo R is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human Thrombopoietin R/Tpo R
Accession # P40238
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human Thrombopoietin R/Tpo R (Catalog # 4444-TR)
Measured by its ability to neutralize Thrombopoietin/Tpo-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 0.5‑2.5 µg/mL in the presence of 10 ng/mL Recombinant Human Thrombopoietin/Tpo.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by Thrombopoietin/Tpo and Neutralization by Human Thrombopoietin R/Tpo R Antibody. View Larger

Cell Proliferation Induced by Thrombopoietin/Tpo and Neutralization by Human Thrombopoietin R/Tpo R Antibody. Recombinant Human Thrombopoietin/Tpo (Catalog # 288-TP) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Thrombopoietin/Tpo (10 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Human Thrombopoietin R/Tpo R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1016). The ND50 is typically 0.5-2.5 µg/mL.

Simple Western View Larger

Detection of Human Thrombopoietin R/Tpo R by Simple WesternTM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Thrombopoietin R/Tpo R at approximately 73 kDa (as indicated) using 20 µg/mL of Goat Anti-Human Thrombopoietin R/Tpo R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombopoietin R/Tpo R

Thrombopoietin receptor (Tpo R), also known as myeloproliferative leukemia protein (c-mpl), is a 95 kDa type I transmembrane protein that is a member of the type I cytokine receptor family within the hematopoietin/cytokine receptor superfamily (1‑4). The 635 amino acid (aa) full-length human Tpo R contains a 25 aa signal sequence, a 466 aa extracellular domain with a ligand binding domain and two fibronectin type III domains, a transmembrane (TM) domain and a cytoplasmic domain. The extracellular domain of human Tpo R shares 78%, 76%, 81%, 82% and 80% aa identity with mouse, rat, bovine, canine and equine Tpo R, respectively. Humans produce three distinct mRNA species; a P-form, a K-form, and a truncated form (Mpl-tr) lacking a TM domain (3‑7). The P-form encodes the full-length receptor. The Mpl-tr form, which is expressed in both human and mouse, is intracellular and targets the P-form for degradation (5, 6). The 579 aa K-form has an alternate cytoplasmic domain, but does not dimerize with, or inhibit, the P-form (7). Thrombopoietin (Tpo) is a key regulator of megakaryocytopoiesis, thrombopoiesis and hematopoietic stem cell self-renewal, as reflected by expression of the Tpo R on megakaryocytes, platelets and hematopoietic progenitors (2, 8). Receptor dimerization occurs upon Tpo binding and initiates signaling through the Ras/MAP and JAK/STAT pathways (1, 2). Internalization and degradation of Tpo following Tpo R binding serves to downregulate circulating Tpo (9). Tpo R expressed at low levels on endothelial cells does not appear to contribute to regulation of Tpo (10). Inactivating mutations of Tpo R cause thrombocytopenia, and absence of functional Tpo R is lethal in humans, but not mice. Other mutations, including an activating change in the TM domain, can cause thrombocytosis (11, 12).

  1. Kaushansky, K. (2005) J. Clin. Invest. 115:3339.
  2. Deutsch, V.R. and A. Tomer (2006) Br. J. Haematol. 134:453. 
  3. Vigon, I. et al. (1992) Proc. Natl. Acad. Sci. USA 89:5640. 
  4. Mignotte, V. et al. (1994) Genomics 20:5. 
  5. Li, J. et al. (2000) Cytokine 12:835. 
  6. Coers, J. et al. (2004) J. Biol. Chem. 279:36397. 
  7. Millot, G.A. et al. (2002) Exp. Hematol. 30:166.
  8. Tong, W. et al. (2007) Exp. Hematol. July 14 [Epub ahead of print].
  9. Li, J. et al. (1999) Br. J. Haematol. 106:345.
  10. Geddis, A.E. et al. (2006) Exp. Hematol. 34:82.
  11. Germeshausen, M. et al. (2006) Hum. Mutat. 27:296.
  12. Ding, J. et al. (2004) Blood 103:4198.
Long Name
Thrombopoietin Receptor
Entrez Gene IDs
4352 (Human); 17480 (Mouse)
Alternate Names
CD110 antigen; CD110; c-Mpl; MPL; MPLV; Myeloproliferative leukemia protein; myeloproliferative leukemia virus oncogene; Proto-oncogene c-Mpl; Thrombopoietin R; thrombopoietin receptor; TpoR; TPO-R; TPORMPLV

Product Datasheets

You must select a language.


Citations for Human Thrombopoietin R/Tpo R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Thrombopoietin participates in platelet activation in COVID-19 patients
    Authors: E Lupia, M Capuano, B Vizio, M Schiavello, O Bosco, M Gelardi, E Favale, E Pivetta, F Morello, S Husain, S Keshavjee, L Del Sorbo, G Montrucchi
    EBioMedicine, 2022;85(0):104305.
    Species: Human
    Sample Types: Plasma
    Applications: Neutralization
  2. Increased Toxoplasma gondii Intracellular Proliferation in Human Extravillous Trophoblast Cells (HTR8/SVneo Line) Is Sequentially Triggered by MIF, ERK1/2, and COX-2
    Authors: ICB Milian, RJ Silva, C Manzan-Mar, BF Barbosa, PM Guirelli, M Ribeiro, A de Oliveir, F Ietta, JR Mineo, P Silva Fran, EAV Ferro
    Front Microbiol, 2019;10(0):852.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  3. BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling.
    Authors: Jeanpierre S, Nicolini FE, Kaniewski B, Dumontet C, Rimokh R, Puisieux A, Maguer-Satta V
    Blood, 2008;112(8):3154-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Thrombopoietin R/Tpo R Antibody

There are currently no reviews for this product. Be the first to review Human Thrombopoietin R/Tpo R Antibody and earn rewards!

Have you used Human Thrombopoietin R/Tpo R Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review