Human TIGIT Antibody (741170)

R&D Systems | Catalog # MAB78981

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Blockade of Receptor-ligand Interaction

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 741170
View all TIGIT Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TIGIT
Met1-Pro141
Accession # Q495A1

Specificity

Detects human TIGIT in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TIGIT Antibody (741170)

Detection of TIGIT by Flow Cytometry

Detection of TIGIT by Flow Cytometry

DNAM-1 Blockade Attenuates and TIGIT Blockade Augments NK-Mediated Cytotoxicity against Adenovirus-Infected CellsThe changes of NK receptors on pNK after exposure to adenovirus-infected TOV21G or their conditioned media were evaluated by flow cytometry. (A) Summary analyses of independent experiments (n = 5) for DNAM-1 (left) and TIGIT (right) expression. (B) Representative images of DNAM-1 (left) and TIGIT (right) expression (Mann-Whitney U tests; CC, co-culture; CM, conditioned-medium). pNK were co-cultured with dl922-947-infected cells with or without a blocking anti-DNAM-1 antibody (10 μg/mL) and assessed by time-lapse microscopy. (C) Representative data of fluorescent object counts over time for TOV21G (left) and summary data (n = 5) of TAUC for TOV21G (right) and (D) OVCAR4 (n = 3) are shown (one-way ANOVA with multiple comparisons test). (E) pNK were co-cultured with OV-infected TOV21G cells with or without a blocking anti-TIGIT antibody (20 μg/mL) and assessed by time-lapse microscopy (n = 5, each line represents a unique donor; also see Figure S8). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32195317), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human TIGIT Antibody (741170)

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 70-350 ng/mL of this antibody will block 50% of the binding of 200 ng/mL of Recombinant Human TIGIT Fc Chimera (Catalog # 7898-TG) to immobilized Recombinant Human CD155/PVR (Catalog # 2530-CD) coated at 2.5 µg/mL (100 µL/well).

Reviewed Applications

Read 1 review rated 4 using MAB78981 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIGIT

TIGIT (T cell Immunoreceptor with Ig and ITIM domains), also called Vstm3 (V-set and transmembrane domain-containing 3), Vsig9 (V-set and Ig domain-containing 9) and WUCAM (Washington University cell adhesion molecule) is a 30-34 kDa type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins. Human TIGIT cDNA encodes 244 amino acids (aa) including a 21 aa signal sequence, a 120 aa extracellular region with a V-type Ig-like domain and two potential N-glycosylation site, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic domain with an ITIM motif. A 170 aa variant diverges after aa 166. Within the ECD, human TIGIT shares only 68-75% aa sequence identity with mouse, porcine, canine, equine and bovine TIGIT. TIGIT is expressed on NK cells and subsets of activated, memory and regulatory T cells, and particularly on follicular helper T cells within secondary lymphoid organs. It binds to CD155/PVR/Necl-5 and Nectin-2/CD112/PVRL2 that appear on dendritic cells (DC) and endothelium. Binding of TIGIT by DC induces IL-10 release and inhibits IL-12 production. Ligation of TIGIT on T cells downregulates TCR-mediated activation and subsequent proliferation, while NK cell TIGIT ligation blocks NK cell cytotoxicity. Through CD155 and Nectin-2, which also interact with DNAM-1/CD226 and CD96/Tactile, TIGIT is part of an interacting network of Ig superfamily members that may augment or oppose each other. In particular, TIGIT binding to CD155 can antagonize the effects of DNAM-1. Soluble TIGIT is able to compete with DNAM-1 for CD155 binding and attenuates T cell responses, while mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges.

Long Name

T Cell Immunoreceptor with Ig and ITIM Domains

Alternate Names

VSIG9, VSTM3, WUCAM

Entrez Gene IDs

201633 (Human); 100043314 (Mouse); 363784 (Rat); 102121588 (Cynomolgus Monkey)

Gene Symbol

TIGIT

UniProt

Q495A1 (Human)

Additional TIGIT Products

Product Documents for Human TIGIT Antibody (741170)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TIGIT Antibody (741170)

For research use only

Citations for Human TIGIT Antibody (741170)

Customer Reviews for Human TIGIT Antibody (741170) (1)

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  • Human TIGIT Antibody
    Name: Anonymous
    Application: Functional Assay
    Sample Tested: Purified protein
    Species: Human
    Verified Customer | Posted 11/09/2018
    Human TIGIT Antibody (741170) MAB78981

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