Human Tissue alpha -L-Fucosidase/FUCA1 Antibody

Catalog # Availability Size / Price Qty
AF7039
AF7039-SP
Detection of Human Tissue  alpha ‑L‑Fucosidase/FUCA1 by Western Blot.
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Human Tissue alpha -L-Fucosidase/FUCA1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Tissue alpha -L-Fucosidase/FUCA1 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Tissue alpha -L-Fucosidase/FUCA1
Gln32-Lys466
Accession # P04066
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Tissue  alpha ‑L‑Fucosidase/FUCA1 by Western Blot. View Larger

Detection of Human Tissue alpha ‑L‑Fucosidase/FUCA1 by Western Blot. Western blot shows lysates of human thyroid tissue and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Tissue alpha ‑L‑Fucosidase/FUCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7039) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tissue alpha ‑L‑Fucosidase/FUCA1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Tissue  alpha ‑L‑Fucosidase/FUCA1 in HeLa Human Cell Line. View Larger

Tissue alpha ‑L‑Fucosidase/FUCA1 in HeLa Human Cell Line. Tissue alpha ‑L‑Fucosidase/FUCA1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Tissue alpha ‑L‑Fucosidase/FUCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7039) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Tissue   alpha ‑L‑Fucosidase/FUCA1 in Human Liver. View Larger

Tissue alpha ‑L‑Fucosidase/FUCA1 in Human Liver. Tissue alpha ‑L‑Fucosidase/FUCA1 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Tissue alpha ‑L‑Fucosidase/FUCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7039) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lysosomes in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tissue alpha-L-Fucosidase/FUCA1

Fucosylated glycoconjugates play numerous roles in biological processes, such as development, apoptosis and the immune responses, and are involved in the pathology of inflammation, cancer and cystic fibrosis (1, 2, 3, 4). Tissue alpha ‑L‑Fucosidase, also known as FUCA1, is a lysosomal enzyme responsible for hydrolyzing alpha ‑L‑fucose moieties from glycolipids and oligosaccharides (5). The mature enzyme in the liver has a molecular weight of 200 kDa and exists in the native state as a tetramer (6). Defects in FUCA1 are the cause of fucosidosis (7, 8, 9), an autosomal recessive lysosomal storage disease characterized by the accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Serum alpha ‑L‑fucosidase has been identified as a useful marker for oral cancer (10).

References
  1. Hiraishi, K. et al. (1993) Glycobiology 3:381.
  2. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
  3. Becker, D.J. and Lowe, J.B. (2003) Glycobiology 13:41R.
  4. Liu, T.W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:14581.
  5. Fukushima, H. et al. (1985) Proc. Natl. Acad. Sci. USA 82:1262.
  6. Alhadeff, J.A. et al. (1975) J. Biol. Chem. 250:7106.
  7. O’Brien, J.S. et al. (1987) Enzyme 38:45.
  8. Durand, P. et al. (1969) J. Pediatr. 75:665.
  9. Johnson, K. and Dawson, G. (1985) Biochem. Biophys. Res. Commun. 133:90.
  10. Shah, M. et al. (2008) Cancer 113:336.
Entrez Gene IDs
2517 (Human); 71665 (Mouse); 24375 (Rat)
Alternate Names
Alpha-L-fucosidase 1; Alpha-L-fucosidase I; Alpha-L-fucoside fucohydrolase 1; EC 3.2.1; EC 3.2.1.51; FUCA; FUCA1; fucosidase, alpha-L- 1, tissue; Tissue alphaLFucosidase; Tissue alpha-L-Fucosidase

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