Human Tissue alpha -L-Fucosidase/FUCA1 Antibody AF7039: R&D Systems

Human Tissue alpha -L-Fucosidase/FUCA1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Tissue alpha -L-Fucosidase/FUCA1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Tissue alpha -L-Fucosidase/FUCA1
    Gln32-Lys466
    Accession # P04066
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Tissue alpha ‑L‑Fucosidase/FUCA1 by Western Blot. Western blot shows lysates of human thyroid tissue and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Tissue alpha ‑L‑Fucosidase/FUCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7039) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tissue alpha ‑L‑Fucosidase/FUCA1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Tissue alpha ‑L‑Fucosidase/FUCA1 in HeLa Human Cell Line. Tissue alpha ‑L‑Fucosidase/FUCA1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Tissue alpha ‑L‑Fucosidase/FUCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7039) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tissue alpha-L-Fucosidase/FUCA1
Fucosylated glycoconjugates play numerous roles in biological processes, such as development, apoptosis and the immune responses, and are involved in the pathology of inflammation, cancer and cystic fibrosis (1, 2, 3, 4). Tissue alpha ‑L‑Fucosidase, also known as FUCA1, is a lysosomal enzyme responsible for hydrolyzing alpha ‑L‑fucose moieties from glycolipids and oligosaccharides (5). The mature enzyme in the liver has a molecular weight of 200 kDa and exists in the native state as a tetramer (6). Defects in FUCA1 are the cause of fucosidosis (7, 8, 9), an autosomal recessive lysosomal storage disease characterized by the accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Serum alpha ‑L‑fucosidase has been identified as a useful marker for oral cancer (10).
  • References:
    1. Hiraishi, K. et al. (1993) Glycobiology 3:381.
    2. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
    3. Becker, D.J. and Lowe, J.B. (2003) Glycobiology 13:41R.
    4. Liu, T.W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:14581.
    5. Fukushima, H. et al. (1985) Proc. Natl. Acad. Sci. USA 82:1262.
    6. Alhadeff, J.A. et al. (1975) J. Biol. Chem. 250:7106.
    7. O’Brien, J.S. et al. (1987) Enzyme 38:45.
    8. Durand, P. et al. (1969) J. Pediatr. 75:665.
    9. Johnson, K. and Dawson, G. (1985) Biochem. Biophys. Res. Commun. 133:90.
    10. Shah, M. et al. (2008) Cancer 113:336.
  • Entrez Gene IDs:
    2517 (Human); 71665 (Mouse); 24375 (Rat)
  • Alternate Names:
    Alpha-L-fucosidase 1; Alpha-L-fucosidase I; Alpha-L-fucoside fucohydrolase 1; EC 3.2.1; EC 3.2.1.51; FUCA; FUCA1; fucosidase, alpha-L- 1, tissue; Tissue alphaLFucosidase; Tissue alpha-L-Fucosidase
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