Human TMED1 Antibody

Catalog # Availability Size / Price Qty
MAB22431-100
MAB22431-SP
Human TMED1 Antibody in Western Blot
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Human TMED1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TMED1 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 1009524
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell, HEK293 derived human TMED1
Met1-Asn194
Accession # Q13445
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human TMED1 by Western Blot. View Larger

Detection of Human TMED1 by Western Blot. Western blot shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TMED1 Monoclonal Antibody (Catalog # MAB22431) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TMED1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of TMED1  in HEK293 Human Cell Line Transfected with Human TMED1 and eGFP by Flow  Cytometry. View Larger

Detection of TMED1 in HEK293 Human Cell Line Transfected with Human TMED1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human TMED1 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human TMED1 Monoclonal Antibody (Catalog # MAB22431) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMED1

TMED1 (Transmembrane Emp24 domain-containing protein 1) is a member of the TMED family of proteins (gene name TMED1). The TMED family of proteins are localized to membranes of the early secretory pathway, including the endoplasmic reticulum and Golgi, and function in vesicular protein trafficking (1, 2). TMED1 is a 59 kDa monomer and has been reported to exist as homodimer (3). TMED1 is composed of a 23 amino acid (aa) signal sequence, a 171 aa extra cellular domain, a 21 aa transmembrane domain, and a 12 aa cytoplasmic domain. The extracellular domain contains an 83 aa GOLD (Golgi Dynamics) domain, and COPI and COPII binding motifs are found in the cytoplasmic domain (1-3, 5). Human TMED1 shares 97% sequence identity with mouse, bovine, and rat homologs within the 171 aa extracellular domain. The beta -strand-rich GOLD domain has been specifically identified to be involved in intracellular protein trafficking (1, 4, 5). TMED1 is important in regulating innate immune signaling through its interaction with ST2L. Specifically, the GOLD domain in TMED1 interacts with the TIR domain of ST2L, a receptor for IL‑33 (1). This interaction promotes ST2L association with IL-33, allowing downstream signaling cascade activating MAP kinases, p38, and JNK (1, 6). Studies have shown knockdown of TMED-1 in HUVECs impairs the IL-33 induced response resulting in reduction of IL-6 and IL-8 productions (1).

References
  1. Connolly, D. et al. (2013) J Biol Chem. 288:5616.
  2. Gour, N. and Lajoie, S. (2018) Curr Allergy Ashma Rep. 16:65.
  3. Jenne, N. (2002) J Biol Chem. 277:46504.
  4. Anantharaman, V. and Aravind, L. (2002) Genome Biol. 3:research0023
  5. Gomez-Navarro, N. and Miller, E. (2016) J Cell Biol. 215:769.
  6. Hardman, C. and Ogg, G. (2016). Curr Opin Immunol. 42:16.
Entrez Gene IDs
11018 (Human); 17083 (Mouse); 315461 (Rat)
Alternate Names
IL1RL1-Binding Protein; Il1rl1l; IL1RL1LG; IL-1RL1LG; IL1RL1LGIL1RL1-binding protein; Interleukin 1 Receptor-Like 1 Ligand; Interleukin-1 receptor-like 1 ligand; Ly84l; MGC1270; P24g1; P24gamma1; Putative T1/ST2 receptor-binding protein; ST2L; T1/ST2 receptor binding protein; TMED1; Tp24; transmembrane emp24 domain containing 1; transmembrane emp24 domain-containing protein 1; transmembrane emp24 protein transport domain containing 1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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