Human TNF-alpha Quantikine QuicKit ELISA Summary
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Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|Intra-Assay Precision||Inter-Assay Precision|
The recovery of human TNF-alpha spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
|Sample Type||Average % Recovery||Range %|
|Cell Culture Media (n=4)||120||108-126|
|EDTA Plasma (n=2)||95||87-106|
|Heparin Plasma (n=2)||89||81-103|
Human TNF- alpha Standard Curve
Human TNF- alpha Standard Curve
Human TNF-alpha ELISA Standard Curve
Human TNF-alpha QuicKit Spiked Recovery Competitor Comparison TNF-alpha is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. Serum recovery is 100% compared to 66% for the top competitor. EDTA plasma recovery is 102% compared to 59% for the top competitor. Heparin plasma recovery is 112% compared to 74% for the top competitor. In spike and recovery experiments, natural samples are spiked with the recombinant target analyte of interest to identify interference caused by sample matrices.
Human TNF-alpha QuicKit Spiked Linearity Competitor Comparison TNF-alpha is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 87%-105% compared to 122%-189% for the top competitor.
Preparation and Storage
Tumor Necrosis Factor alpha (TNF-alpha ), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily (1). It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (2-5). TNF-alpha is also involved in a number of pathological conditions including asthma, Crohn's disease, rheumatoid arthritis, neuropathic pain, obesity, type 2 diabetes, septic shock, autoimmunity, and cancer (5-11).
These assays utilize an anti-tag coated microplate. Standards, samples, and controls are added to plate, followed by subsequent addition of an antibody cocktail. Following a 1 hour incubation, plates are washed and substrate is added and incubated for 20 minutes. Stop solution is then added and plates are read using a standard microplate reader.
Citations for Human TNF-alpha Quantikine QuicKit ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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LINC01094/SPI1/CCL7 Axis Promotes Macrophage Accumulation in Lung Adenocarcinoma and Tumor Cell Dissemination
Authors: Z Wu, X Bai, Z Lu, S Liu, H Jiang
Journal of Immunology Research, 2022;2022(0):6450721.
Sample Types: Cell Culture Supernates
Effect of mechanical instrumentation with adjunct photodynamic therapy on salivary TNFalpha levels and clinical periodontal and peri-implant status in patients with depression: A randomized controlled trial
Authors: SS ALHarthi, DD Divakar, A Alwahibi, MS BinShabaib
Sample Types: Saliva
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