Human TNF-alpha Quantikine QuicKit ELISA Summary
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Natural and recombinant human TNF-alpha.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.
The Quantikine® QuicKit™ Human TNF-alpha Immunoassay is a one step, 80 minute solid phase ELISA designed to measure human TNF-alpha in cell culture supernates, serum, and plasma. It contains E.
coli-expressed recombinant human TNF-alpha and antibodies raised against the recombinant protein. Results obtained for naturally occurring human TNF-alpha showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit™ standards. These results indicate that this kit can be used to determine relative mass values for natural human TNF-alpha.
Intra-Assay Precision (Precision within an assay) <div>Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.</div>
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least three technicians
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
The recovery of human TNF-alpha spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
||Average % Recovery
|Cell Culture Media (n=4)
|EDTA Plasma (n=2)
|Heparin Plasma (n=2)
To assess linearity of the assay, samples containing and/or spiked with high concentrations of human TNF-alpha in various matrices were diluted with the calibrator diluent to produce samples with values within the dynamic range of the assay.
Human TNF- alpha Standard Curve
Human TNF- alpha Standard Curve
Human TNF-alpha ELISA Standard Curve
Human TNF-alpha QuicKit Spiked Recovery Competitor Comparison
TNF-alpha is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. Serum recovery is 100% compared to 66% for the top competitor. EDTA plasma recovery is 102% compared to 59% for the top competitor. Heparin plasma recovery is 112% compared to 74% for the top competitor. In spike and recovery experiments, natural samples are spiked with the recombinant target analyte of interest to identify interference caused by sample matrices.
Human TNF-alpha QuicKit Spiked Linearity Competitor Comparison
TNF-alpha is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 87%-105% compared to 122%-189% for the top competitor.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Tumor Necrosis Factor alpha (TNF-alpha ), also known as cachectin and TNFSF1A, is the prototypic
ligand of the TNF superfamily (1). It is a pleiotropic molecule that plays a central role in
inflammation, immune system development, apoptosis, and lipid metabolism (2-5). TNF-alpha
is also involved in a number of pathological conditions including asthma, Crohn's disease,
rheumatoid arthritis, neuropathic pain, obesity, type 2 diabetes, septic shock, autoimmunity,
and cancer (5-11).
Human TNF-alpha is synthesized as a 26 kDa type II transmembrane protein that consists of
a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa
extracellular domain (ECD) (12, 13). Within the ECD, human TNF-alpha shares 97% aa sequence
identity with rhesus monkey, and 71%-92% aa identity with bovine, canine, cotton rat, equine,
feline, mouse, porcine, and rat TNF-alpha. It is produced by a wide variety of immune, epithelial,
endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked
homotrimer which is expressed on the cell surface (14). Cell surface TNF-alpha can both induce the
lysis of tumor cells and virus infected cells, and generate its own downstream cell signaling
following ligation by soluble TNF RI (15, 16). Shedding of membrane bound TNF-alpha by TACE/
ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular
TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (20, 21) and the hematopoietic cell-restricted
78-80 kDa TNF RII (22, 23), both of which are also expressed as homotrimers (1, 24). Both
type I and type II receptors bind TNF-alpha with comparable affinity and can promote NF kappa B
activation (25-28). Only TNF RI, however, contains a cytoplasmic death domain which triggers
the activation of apoptosis (3, 29). Soluble forms of both types of receptors are released into
human serum and urine and can neutralize the biological activity of TNF-alpha (30-32).
Tumor Necrosis Factor alpha
Entrez Gene IDs:
7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha