Human TNF RI/TNFRSF1A Antibody

Name: Human TNF RI/TNFRSF1A Antibody
Brand: R&D Systems
SKU: MAB225R
Availability: InStock

R&D Systems | Catalog # MAB225R

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Product Documents

Recombinant Monoclonal Antibody.

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG₁ Clone # 16803R

View all TNF RI/TNFRSF1A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TNF RI/TNFRSF1A
Accession # NP_001056

Specificity

Detects human TNF R1 in Western blots. In Western blots, no cross-reactivity with recombinant human TNF RII, recombinant mouse (rm) TNF RI, or rmTNF RII is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human TNF RI/TNFRSF1A Antibody

Detection of TNF RI/TNFRSF1A in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Recombinant Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB225R, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

TNF RI/TNFRSF1A Inhibition of TNF-alpha induced Cytotoxicity and Neutralization by Human TNF RI/TNFRSF1A Antibody.

Recombinant Human TNF RI/TNFRSF1A (Catalog # 372-R1) inhibits Recombinant Human TNF-a (Catalog # 210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human TNF-a (0.25 ng/mL) activity elicited by Recombinant Human TNF RI/TNFRSF1A (15 ng/mL) is neutralized (green line) by increasing concentrations of Human TNF RI/TNFRSF1A Recombinant Monoclonal Antibody (Catalog # MAB225R). The ND₅₀ is typically 0.008-0.04 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

Applications for Human TNF RI/TNFRSF1A Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood monocytes

Neutralization

Measured by its ability to neutralize TNF RI/TNFRSF1A-mediated inhibition of cytotoxicity in the L-929 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.008-0.04 μg/mL in the presence of 15 ng/mL Recombinant Human TNF RI/TNFRSF1A, 0.25 ng/mL Recombinant Human TNF-alpha, and 1 μg/mL actinomycin D.

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TNF RI/TNFRSF1A

TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.