Detects human TNF R1 in Western blots. In Western blots, no cross-reactivity with recombinant human TNF RII, recombinant mouse (rm) TNF RI, or rmTNF RII is observed.
Recombinant Monoclonal Mouse IgG1 Clone # 16803R
Protein A or G purified from cell culture supernatant
E. coli-derived recombinant human TNF RI/TNFRSF1A Accession # NP_001056
as a solution in PBS. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
0.25 µg/106 cells
Measured by its ability to neutralize TNF RI/TNFRSF1A-mediated inhibition of cytotoxicity in the L-929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.008-0.04 μg/mL in the presence of 15 ng/mL Recombinant Human TNF RI/TNFRSF1A, 0.25 ng/mL Recombinant Human TNF-alpha, and 1 μg/mL actinomycin D.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
TNF RI/TNFRSF1A Inhibition of TNF-alpha induced Cytotoxicity and Neutralization by Human TNF RI/TNFRSF1A Antibody. Recombinant Human TNF RI/TNFRSF1A (Catalog # 372-R1) inhibits Recombinant Human TNF-alpha (Catalog # 210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human TNF-alpha (0.25 ng/mL) activity elicited by Recombinant Human TNF RI/TNFRSF1A (15 ng/mL) is neutralized (green line) by increasing concentrations of Human TNF RI/TNFRSF1A Recombinant Monoclonal Antibody (Catalog # MAB225R). The ND50 is typically 0.008-0.04 μg/mL in the presence of the metabolic inhibitor actinomycin D (1 μg/mL).
Detection of TNF RI/TNFRSF1A in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Recombinant Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB225R, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
Background: TNF RI/TNFRSF1A
TNF RI occurs both in membrane bound and soluble forms and functions as
a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is
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