Human TOB1 Antibody AF5228: R&D Systems

Human TOB1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human TOB1 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human TOB1
    Met1-Asn345
    Accession # P50616
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human TOB1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Human TOB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5228) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TOB1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TOB1

TOB1 (transducer of ErbB2 protein 1) is a 45 kDa member of the BTG family of anti‑proliferative proteins. It is ubiquitously expressed, and serves to negatively regulate cell cycle progression from G0/G1 to S phase. This is accomplished by TOB1 suppression of cyclin D1 expression, which is abrogated by Erk2-mediated phosphorylation of Ser152/154/164 in response to growth factor stimulation. Human TOB1 is 345 amino acids (aa) in length and contains one NES (aa 2‑14), a bipartite NLS (aa 18‑40), a BTG domain (aa 42‑130) that possesses antiproliferative activity, and two PAM domains (aa 133‑143 and 268‑278) that bind inducible PABP and regulate gene transcription. Human and mouse TOB1 (aa 1‑345) are 94% aa identical when fourteen mouse-specific glutamines are excluded from the alignment.

  • Long Name:
    Transducer of ERBB2 Protein 1
  • Entrez Gene IDs:
    10140 (Human); 22057 (Mouse); 170842 (Rat)
  • Alternate Names:
    APRO6; MGC104792; PIG49; protein Tob1; TOB1; TOBMGC34446; Transducer of erbB-2 1; transducer of ERBB2, 1; TROB; TROB1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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