Human TOB1 Antibody

Catalog # Availability Size / Price Qty
AF5228
AF5228-SP
Human TOB1 Antibody in Data
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Human TOB1 Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human TOB1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TOB1
Met1-Asn345
Accession # P50616
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human TOB1 antibody by Western Blot. View Larger

Detection of Human TOB1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Human TOB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5228) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TOB1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TOB1

TOB1 (transducer of ErbB2 protein 1) is a 45 kDa member of the BTG family of anti‑proliferative proteins. It is ubiquitously expressed, and serves to negatively regulate cell cycle progression from G0/G1 to S phase. This is accomplished by TOB1 suppression of cyclin D1 expression, which is abrogated by Erk2-mediated phosphorylation of Ser152/154/164 in response to growth factor stimulation. Human TOB1 is 345 amino acids (aa) in length and contains one NES (aa 2‑14), a bipartite NLS (aa 18‑40), a BTG domain (aa 42‑130) that possesses antiproliferative activity, and two PAM domains (aa 133‑143 and 268‑278) that bind inducible PABP and regulate gene transcription. Human and mouse TOB1 (aa 1‑345) are 94% aa identical when fourteen mouse-specific glutamines are excluded from the alignment.

Long Name
Transducer of ERBB2 Protein 1
Entrez Gene IDs
10140 (Human); 22057 (Mouse); 170842 (Rat)
Alternate Names
APRO6; MGC104792; PIG49; protein Tob1; TOB1; TOBMGC34446; Transducer of erbB-2 1; transducer of ERBB2, 1; TROB; TROB1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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