|Detection of Human TOB1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Human TOB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5228) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TOB1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
TOB1 (transducer of ErbB2 protein 1) is a 45 kDa member of the BTG family of anti‑proliferative proteins. It is ubiquitously expressed, and serves to negatively regulate cell cycle progression from G0/G1 to S phase. This is accomplished by TOB1 suppression of cyclin D1 expression, which is abrogated by Erk2-mediated phosphorylation of Ser152/154/164 in response to growth factor stimulation. Human TOB1 is 345 amino acids (aa) in length and contains one NES (aa 2‑14), a bipartite NLS (aa 18‑40), a BTG domain (aa 42‑130) that possesses antiproliferative activity, and two PAM domains (aa 133‑143 and 268‑278) that bind inducible PABP and regulate gene transcription. Human and mouse TOB1 (aa 1‑345) are 94% aa identical when fourteen mouse-specific glutamines are excluded from the alignment.
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