TORC3 in HeLa Human Cell Line.
TORC3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human TORC3 Monoclonal Antibody (Catalog # MAB9455) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CREB-Regulated Transcription Coactivator 3 (CRTC3), also
known as TORC3, a transcriptional coactivator for CREB1, acts as a coactivator
in the SIK/TORC signaling pathway. Dephosphorylated CRTC3 increases the
interaction of CREB1 cAMP response element (CRE) sites. CRTC3 activity is
important in regulating the expression of genes involved in cellular energy
metabolism. Its binding to the HTLV-1 protein Tax enhances HTLV-1
transcription, whereas its binding to cellular Bcl3 inhibits HTLV-1
transcription. CRTC3 polymorphism have
been associated with obesity and hypertriglyceridemia.
Transducer of Regulated CREB-binding Protein 3
Entrez Gene IDs:
64784 (Human); 70461 (Mouse); 365297 (Rat)
CREB regulated transcription coactivator 3; CRTC3; FLJ21868; TORC3; TORC-3; TORC3CREB-regulated transcription coactivator 3; Transducer of CREB protein 3; transducer of regulated cAMP response element-binding protein (CREB) 3; Transducer of regulated cAMP response element-binding protein 3; transducer of regulated CREB protein 3
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