Human TRAF-1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human TRAF‑1 by Western Blot.
1 Image
Product Details
Supplemental Products

Human TRAF-1 Antibody Summary

Species Reactivity
Detects human TRAF-1 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human TRAF-2, -3, -4, -5, and -6 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human TRAF-1
Accession # Q13077
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TRAF‑1 antibody by Western Blot. View Larger

Detection of Human TRAF‑1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human TRAF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3276) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional refer-ence, recombinant human TRAF-1, TRAF-2, TRAF-3, TRAF-4, TRAF-5, and TRAF-6 (2 ng/lane) were included. A specific band for TRAF-1 was detected at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAF-1

Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL-1 receptor family members and mediate cellular signaling. Six members of the TRAF family have been identified. All TRAF proteins have a homologous C-terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAF-1, also known as EBI6, is a 416 amino acid, 46 kDa protein that is a unique member of the TRAF family, in that it lacks the N-terminal RING finger domain common in TRAFs 2-6. TRAF-1 interacts with the cytoplasmic domain of TNFR2 and other TNFR family members to mediate downstream signaling events. TRAF-1 is a substrate for caspases activated by TNF family death receptors. TRAF-1 can homodimerize as well as form heterodimers with TRAF-2.

Long Name
TNF Receptor-Associated Factor 1
Entrez Gene IDs
7185 (Human); 22029 (Mouse)
Alternate Names
EBI6; EBI6MGC:10353; Epstein-Bar virus-induced protein 6; Epstein-Barr virus-induced protein 6; TNF receptor-associated factor 1; TRAF1; TRAF-1

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human TRAF-1 Antibody

There are currently no reviews for this product. Be the first to review Human TRAF-1 Antibody and earn rewards!

Have you used Human TRAF-1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review