Detection of Human Transthyretin/Prealbumin by Western Blot.
Western blot shows human plasma. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Transthyretin/Prealbumin Monoclonal Antibody (Catalog # MAB7505) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Transthyretin/Prealbumin at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Transthyretin/Prealbumin in HepG2 Human Cell Line.
Transthyretin/Prealbumin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Transthyretin/ Prealbumin Monoclonal Antibody (Catalog # MAB7505) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
previously called thyroxin-binding prealbumin, is a 55 kDa homotetramer of 14-15 kDa monomers that is found
in plasma. TTR is responsible for the transport of the thyroid hormone T4 and
retinol binding protein (RBP). It slows renal clearance of T4 and RBP due to
megalin-mediated re-uptake of TTR complexes in the renal tubules. TTR is also
found in tissues including the brain. Numerous human polymorphisms have been
associated with aggregates found in familial amyloidotic polyneuropathies
(FAP). Mature human TTR shares 80% and 83% amino acid sequence identity with
mouse and rat TTR, respectively.
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