Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Porcine

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 760430
View all Transthyretin/Prealbumin Primary Antibodies »

Product Specifications

Immunogen

Human Transthyretin/Prealbumin

Specificity

Detects human Transthyretin/Prealbumin in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Transthyretin/Prealbumin Antibody

Detection of Human Transthyretin/Prealbumin antibody by Western Blot.

Detection of Human Transthyretin/Prealbumin by Western Blot.

Western blot shows human plasma. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Transthyretin/Prealbumin Monoclonal Antibody (Catalog # MAB7505) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Transthyretin/Prealbumin at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Transthyretin/Prealbumin antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

Transthyretin/Prealbumin in HepG2 Human Cell Line.

Transthyretin/Prealbumin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Transthyretin/ Prealbumin Monoclonal Antibody (Catalog # MAB7505) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Transthyretin/Prealbumin by Immunohistochemistry

Detection of Transthyretin/Prealbumin by Immunohistochemistry

ChP cells are efficiently generated in IVD‐organoids. (A) Immunostaining of TTR in IVD‐organoids, dissociated cells and day 60 hCOs. Scale bars, 50 μm. Quantification results are presented as mean ± s.e.m (n = 3 for transplanted mice, dissociated cells and cultured hCOs). (B) Integrated UMAP plots showing joint clustering of IVD‐organoids and hCOs. Colored by samples (left) and cell types (right). (C) Violin plots showing expression levels of mature ChP markers in hCOs and IVD‐organoids. (D) Violin plots showing expression levels of tight junction markers in hCOs and IVD‐organoids. (E) PCA analysis of the ChP cell cluster in IVD‐organoids and various cell clusters in ChP organoids Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35141969), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Transthyretin/Prealbumin Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Western Blot

2 µg/mL
Sample: Human plasma

Reviewed Applications

Read 1 review rated 4 using MAB7505 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Transthyretin/Prealbumin

Transthyretin (TTR), previously called thyroxin-binding prealbumin, is a 55 kDa homotetramer of 14-15 kDa monomers that is found in plasma. TTR is responsible for the transport of the thyroid hormone T4 and retinol binding protein (RBP). It slows renal clearance of T4 and RBP due to megalin-mediated re-uptake of TTR complexes in the renal tubules. TTR is also found in tissues including the brain. Numerous human polymorphisms have been associated with aggregates found in familial amyloidotic polyneuropathies (FAP). Mature human TTR shares 80% and 83% amino acid sequence identity with mouse and rat TTR, respectively.

Alternate Names

ATTR, PALB, TBPA, TTR

Entrez Gene IDs

7276 (Human)

Gene Symbol

TTR

Additional Transthyretin/Prealbumin Products

Product Documents for Human Transthyretin/Prealbumin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Transthyretin/Prealbumin Antibody

For research use only

Citations for Human Transthyretin/Prealbumin Antibody

Customer Reviews for Human Transthyretin/Prealbumin Antibody (1)

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  • Name: Anonymous
    Application: Simple Western
    Sample Tested: SVG-A and SH-SY5Y cells
    Species: Human
    Verified Customer | Posted 03/31/2016
    TTR expression in SVG-A and SH-SY5Y cells
    Human Transthyretin/Prealbumin Antibody MAB7505

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Protocols

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