Human Trefoil Factor 3 Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human TFF3 in direct ELISAs and Western blots. Does not cross-react with recombinant human TFF2.
  • Source
    Monoclonal Mouse IgG1 Clone # 415909
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TFF3
    Ala21-Phe80
    Accession # Q07654
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human TFF3
    under non-reducing conditions only
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
TFF3 in Human Colon. TFF3 was detected in immersion fixed paraffin-embedded sections of human colon using 25 µg/mL Human TFF3 Monoclonal Antibody (Catalog # MAB4407) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of goblet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TFF3

Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes. Trefoils contribute to protection and repair of the gastrointestinal tract. TFF3 is secreted by mucus-producing goblet cells in the large and small intestines and lower respiratory tract. It is mainly active as a disulfide-linked homodimer. The mature 59 aa, 6.5 kDa human TFF3 contains one trefoil structure formed by intramolecular disulfides and shows 76% aa identity with mouse TFF3.

  • Long Name:
    Trefoil Factor 3
  • Entrez Gene IDs:
    7033 (Human); 21786 (Mouse); 25563 (Rat)
  • Alternate Names:
    HITF, human intestinal trefoil factor10Intestinal trefoil factor; ITF; TFI; trefoil factor 3 (intestinal)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Species
Applications
Sample Type
  1. Non-endoscopic screening biomarkers for Barrett's oesophagus: from microarray analysis to the clinic.
    Authors: Lao-Sirieix P, Boussioutas A, Kadri SR, O'Donovan M, Debiram I, Das M, Harihar L, Fitzgerald RC
    Gut, 2009;58(11):1451-9.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  2. Trefoil factor 3: a novel serum marker identified by gene expression profiling in high-grade endometrial carcinomas.
    Authors: Bignotti E, Ravaggi A, Tassi RA, Calza S, Rossi E, Falchetti M, Romani C, Bandiera E, Odicino FE, Pecorelli S, Santin AD
    Br. J. Cancer, 2008;99(5):768-73.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

CTS002 8
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Anti-Mouse HRP-AEC Cell & Tissue Staining Kit

CTS003 4
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 6
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Antigen Retrieval Reagent-Basic

CTS013 3
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Mounting Medium

CTS011 1
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Antigen Retrieval Reagent-Acidic

CTS014
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DAB Enhancer

CTS010 1
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NorthernLights Guard Mounting Media

NL996
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Isotype Controls
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Mouse IgG1 Isotype Control

Ctrl MAB002 95  
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Mouse IgG1 Isotype Control

Ctrl MAB002R
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Molecular Weight Markers
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Full-Range Molecular Weight Marker, Biotinylated

MW001
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Secondary Antibodies
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Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF007 14  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 8  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL007 8
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 6
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 4
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF018 2  
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Goat Anti-Mouse IgG Antibody

WB AF007 3
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 2  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 2
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 1
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018
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