Human Trefoil Factor 3 Antibody

Catalog # Availability Size / Price Qty
MAB4407
MAB4407-SP
TFF3 in Human Colon.
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (5)

Human Trefoil Factor 3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TFF3 in direct ELISAs and Western blots. Does not cross-react with recombinant human TFF2.
Source
Monoclonal Mouse IgG1 Clone # 415909
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TFF3
Ala21-Phe80
Accession # Q07654
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TFF3
under non-reducing conditions only
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry TFF3 in Human Colon. View Larger

TFF3 in Human Colon. TFF3 was detected in immersion fixed paraffin-embedded sections of human colon using 25 µg/mL Human TFF3 Monoclonal Antibody (Catalog # MAB4407) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of goblet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFF3

Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes. Trefoils contribute to protection and repair of the gastrointestinal tract. TFF3 is secreted by mucus-producing goblet cells in the large and small intestines and lower respiratory tract. It is mainly active as a disulfide-linked homodimer. The mature 59 aa, 6.5 kDa human TFF3 contains one trefoil structure formed by intramolecular disulfides and shows 76% aa identity with mouse TFF3.

Long Name
Trefoil Factor 3
Entrez Gene IDs
7033 (Human); 21786 (Mouse); 25563 (Rat)
Alternate Names
HITF, human intestinal trefoil factor10Intestinal trefoil factor; ITF; TFF3; TFI; trefoil factor 3 (intestinal)

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Citations for Human Trefoil Factor 3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Non-endoscopic screening biomarkers for Barrett's oesophagus: from microarray analysis to the clinic.
    Authors: Lao-Sirieix P, Boussioutas A, Kadri SR, O'Donovan M, Debiram I, Das M, Harihar L, Fitzgerald RC
    Gut, 2009;58(11):1451-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. Trefoil factor 3: a novel serum marker identified by gene expression profiling in high-grade endometrial carcinomas.
    Authors: Bignotti E, Ravaggi A, Tassi RA, Calza S, Rossi E, Falchetti M, Romani C, Bandiera E, Odicino FE, Pecorelli S, Santin AD
    Br. J. Cancer, 2008;99(5):768-73.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Trefoil Factor 3 Antibody

Average Rating: 4.4 (Based on 5 Reviews)

5 Star
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Human Trefoil Factor 3 Antibody
By Anonymous on 10/27/2021
Application: IHC Sample Tested: Colon tissue Species: Human

Human Trefoil Factor 3 Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Trefoil Factor 3 Antibody
By Anonymous on 02/07/2020
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma


Human Trefoil Factor 3 Antibody
By Anonymous on 11/08/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human and Mouse

Human Trefoil Factor 3 Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human