Human TrkA Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB1751R-100
MAB1751R-SP
Detection of TrkA in BAF Cell Line Transfected with Human TrkA and eGFP by Flow Cytometry.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human TrkA Antibody Summary

Species Reactivity
Human
Specificity
Detects human TrkA in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 165131R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TrkA
Ala33-Glu407
Accession # P04629
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TrkA in BAF Cell Line Transfected with Human TrkA and eGFP by Flow Cytometry. View Larger

Detection of TrkA in BAF Cell Line Transfected with Human TrkA and eGFP by Flow Cytometry. BAF cell line transfected with human TrkA and eGFP was stained with (A) Mouse Anti-Human TrkA Monoclonal Antibody (Catalog # MAB1751R) or (B) Mouse IgG1 isotype control antibody (Catalog # MAB002) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TrkA

TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, TrkB, and TrkC preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct TrkA isoforms that differ by virtue of a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA is expressed mainly in non-neuronal tissues. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of TrkA with the p75 NGF receptor (NGF R), a member of the tumor necrosis factor receptor superfamily. NGF R binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGF R can also mediate cell death when expressed independent of Trk.

References
  1. Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
  2. Sofroniew, M.V. et al. (200) Annu. Rev. Neurosci. 24:1217.
Long Name
Neurotrophic Tyrosine Kinase Receptor A
Entrez Gene IDs
4914 (Human); 59109 (Rat)
Alternate Names
DKFZp781I14186; EC 2.7.10; EC 2.7.10.1; MTChigh affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase, receptor, type 1; NTRK1; NTRK-1; p140-TrkA; TRK1-transforming tyrosine kinase protein; TrkA; Trk-A; TRKAOncogene TRK; TRKTRK1; tyrosine kinase receptor A

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Mouse IgG1 Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Reviews for Human TrkA Antibody

There are currently no reviews for this product. Be the first to review Human TrkA Antibody and earn rewards!

Have you used Human TrkA Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review