Human Troponin I Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8594
MAB8594-SP
Detection of Human, Mouse, and Rat Troponin I by Western Blot.
3 Images
Product Details
FAQs
Supplemental Products
Reviews

Human Troponin I Antibody Summary

Species Reactivity
Human
Specificity
Detects human Troponin I in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1188B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Peptide 3
Accession # P19429
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below
Simple Western
1:100 dilution
See below
Immunocytochemistry
1:100 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat Troponin I by Western Blot. View Larger

Detection of Human, Mouse, and Rat Troponin I by Western Blot. Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Troponin I Monoclonal Antibody (Catalog # MAB8594) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Troponin I at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Troponin I in Human Cardiomyocytes. View Larger

Troponin I in Human Cardiomyocytes. Troponin I was detected in immersion fixed human embryonic stem cells, differentiated into cardiomyocytes using the StemXVivo Cardiomyocyte Differentiation Kit (Catalog # SC032), using Rabbit Anti-Human Troponin I Monoclonal Antibody (Catalog # MAB8594) at 1:100 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006). Cardiac Troponin T was also detected in cardiomyocytes using Mouse Anti-Human Cardiac Troponin T Monoclonal Antibody (Catalog # MAB1874). Cells were co-stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining of Troponin I was localized to cardiac muscle fibrils. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human Troponin I by Simple WesternTM. View Larger

Detection of Human Troponin I by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.1 mg/mL. A specific band was detected for Troponin I at approximately 25-35 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human Troponin I Monoclonal Antibody (Catalog # MAB8594). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: Troponin I

Troponin I, also known as TNI, is a 24 kDa component of a protein complex on striated muscle thin filaments. Troponin I inhibits the calcium-dependent muscle contraction mediated by Troponins C and T. The expression of cardiac Troponin I (TNNI3) is restricted to cardiac muscle, while TNNI1 and TNNI2 (encoded by distinct genes) are expressed in skeletal muscle. Mutations of cardiac Troponin I are associated with heriditary cardiomyopathy. Human cardiac Troponin I shares 93% amino acid sequence identity with mouse and rat cardiac Troponin I.

Entrez Gene IDs
7137 (Human); 21954 (Mouse); 29248 (Rat)
Alternate Names
CMH7; TNNC1; TNNI3; Troponin I

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Secondary Antibodies

Reviews for Human Troponin I Antibody

There are currently no reviews for this product. Be the first to review Human Troponin I Antibody and earn rewards!

Have you used Human Troponin I Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review