Human Troponin I/TNNI3 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8594
MAB8594-SP
Troponin I/TNNI3 in Human Cardiomyocytes.
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Human Troponin I/TNNI3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Troponin I/TNNI3 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1188B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Peptide 3
Accession # P19429
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry Troponin I antibody in Human Cardiomyocytes by Immunocytochemistry (ICC). View Larger

Troponin I/TNNI3 in Human Cardiomyocytes. Troponin I/TNNI3 was detected in immersion fixed human embryonic stem cells, differentiated into cardiomyocytes using the StemXVivo Cardiomyocyte Differentiation Kit (Catalog # SC032), using Rabbit Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB8594) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006). Cardiac Troponin T was also detected in cardiomyocytes using Mouse Anti-Human Cardiac Troponin T Monoclonal Antibody (Catalog # MAB1874). Cells were co-stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining of Troponin I/TNNI3 was localized to cardiac muscle fibrils. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot Detection of Human, Mouse, and Rat Troponin I antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Troponin I/TNNI3 by Western Blot. Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB8594) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Troponin I/TNNI3 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Troponin I antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Troponin I/TNNI3 by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.1 mg/mL. A specific band was detected for Troponin I/TNNI3 at approximately 25-35 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB8594). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Troponin I/TNNI3

Troponin I, also known as TNI, is a 24 kDa component of a protein complex on striated muscle thin filaments. Troponin I inhibits the calcium-dependent muscle contraction mediated by Troponins C and T. The expression of cardiac Troponin I (TNNI3) is restricted to cardiac muscle, while TNNI1 and TNNI2 (encoded by distinct genes) are expressed in skeletal muscle. Mutations of cardiac Troponin I are associated with heriditary cardiomyopathy. Human cardiac Troponin I shares 93% amino acid sequence identity with mouse and rat cardiac Troponin I.

Long Name
Troponin I3, Cardiac Type
Entrez Gene IDs
7137 (Human)
Alternate Names
Cardiac troponin I; CMD1FF; CMD2A; CMH7MGC116817; CTnI; familial hypertrophic cardiomyopathy 7; RCM1; TNNC1; TNNC1troponin I, cardiac; TNNI3,; troponin I type 3 (cardiac); troponin I, cardiac muscle; Troponin I/TNNI3

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