Human Troponin I/TNNI3 Antibody Summary
Accession # P19429
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat Troponin I/TNNI3 by Western Blot. Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB8594) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Troponin I/TNNI3 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Troponin I/TNNI3 by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.1 mg/mL. A specific band was detected for Troponin I/TNNI3 at approximately 25-35 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB8594). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Troponin I/TNNI3 in Human Cardiomyocytes. Troponin I/TNNI3 was detected in immersion fixed human embryonic stem cells, differentiated into cardiomyocytes using the StemXVivo Cardiomyocyte Differentiation Kit (Catalog # SC032), using Rabbit Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB8594) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006). Cardiac Troponin T was also detected in cardiomyocytes using Mouse Anti-Human Cardiac Troponin T Monoclonal Antibody (Catalog # MAB1874). Cells were co-stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining of Troponin I/TNNI3 was localized to cardiac muscle fibrils. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Troponin I/TNNI3
Troponin I, also known as TNI, is a 24 kDa component of a protein complex on striated muscle thin filaments. Troponin I inhibits the calcium-dependent muscle contraction mediated by Troponins C and T. The expression of cardiac Troponin I (TNNI3) is restricted to cardiac muscle, while TNNI1 and TNNI2 (encoded by distinct genes) are expressed in skeletal muscle. Mutations of cardiac Troponin I are associated with heriditary cardiomyopathy. Human cardiac Troponin I shares 93% amino acid sequence identity with mouse and rat cardiac Troponin I.
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