Human ULBP-3 Antibody

Catalog # Availability Size / Price Qty
AF1517
AF1517-SP
Product Details
Citations (6)
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Human ULBP-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ULBP-3 in direct ELISAs and Western blots. In these formats, approximately 5% cross‑reactivity with recombinant human (rh) ULBP-2 is observed and less than 1% cross-reactivity with rhULBP-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ULBP-3
Gly27-Pro216
Accession # NP_078794
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human ULBP‑3 Fc Chimera (Catalog # 1517-UL)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-3 µg/mL of this antibody will block 50% of the binding of 20 ng/mL of biotinylated Recombinant Human ULBP-3 Fc Chimera to immobilized Recombinant Human NKG2D Fc Chimera (Catalog # 1299-NK) coated at 2 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ULBP-3

ULBP-3 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-3 has also been described under the names RaeT1N (retinoic acid early transcript), NKG2DL3, and ALCAN-gamma. The name ULBP-3 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-3 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30-60%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin. Some family members, including ULBP-3, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. ULBP‑3 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance (1-7).

References
  1. Cosman, D. et al. (2001) Immunity 14:123.
  2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
  3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
  4. Steinle, A. et al. (2001) Immunogenetics 53:279.
  5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Radosavljevic, M. et al. (2002) Genomics 79:114.
  8. NKG2D and its Ligands (2002) http://www.RnDSystems.com/.
Long Name
UL16 Binding Protein-3
Entrez Gene IDs
79465 (Human)
Alternate Names
NKG2D ligand 3; RAET1N; UL16 binding protein 3; ULBP3; ULBP-3

Product Datasheets

Citations for Human ULBP-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Natural Killer Group 2D Ligand Depletion Reconstitutes Natural Killer Cell Immunosurveillance of Head and Neck Squamous Cell Carcinoma
    Authors: S Weil, S Memmer, A Lechner, V Huppert, A Giannattas, T Becker, A Müller-Run, K Lampe, D Beutner, A Quaas, R Schubert, E Herrmann, A Steinle, U Koehl, L Walter, MS von Bergwe, J Koch
    Front Immunol, 2017;8(0):387.
    Applications: ELISA Development (Capture)
  2. Natural Killer Cell Evasion Is Essential for Infection by Rhesus Cytomegalovirus
    PLoS Pathog, 2016;12(8):e1005868.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Immunohistochemical validation and expression profiling of NKG2D ligands in a wide spectrum of human epithelial neoplasms.
    Authors: Fujita H, Hatanaka Y, Sutoh Y, Suzuki Y, Oba K, Hatanaka K, Mitsuhashi T, Otsuka N, Fugo K, Kasahara M, Matsuno Y
    J Histochem Cytochem, 2015;63(3):217-27.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids.
    Authors: Giannattasio A, Weil S, Kloess S, Ansari N, Stelzer E, Cerwenka A, Steinle A, Koehl U, Koch J
    BMC Cancer, 2015;15(0):351.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  5. The human NKG2D ligand ULBP2 can be expressed at the cell surface with or without a GPI anchor and both forms can activate NK cells.
    Authors: Fernandez-Messina L, Ashiru O, Aguera-Gonzalez S, Reyburn HT, Vales-Gomez M
    J. Cell. Sci., 2011;124(0):321-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. NKG2D ligand expression in human colorectal cancer reveals associations with prognosis and evidence for immunoediting.
    Authors: McGilvray RW, Eagle RA, Watson NF, Al-Attar A, Ball G, Jafferji I, Trowsdale J, Durrant LG
    Clin. Cancer Res., 2009;15(22):6993-7002.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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