Human ULBP-4/RAET1E Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human ULBP‑4/RAET1E in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) ULBP-1, rhULBP-2, and rhULBP-3 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human ULBP‑4/RAET1E
    Gly30-Asp225
    Accession # Q8TD07
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human ULBP‑4/RAET1E by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human ULBP‑4/RAET1E Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6285) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ULBP‑4/RAET1E at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ULBP-4/RAET1E

ULBP-4 (UL16 binding protein 4; also NKG2D Ligand 4, Latel and RAET1E) is a 30-40 kDa glycoprotein member of the MHC Class I family of molecules. It has widespread tissue expression, and is particularly prominent on tumor cells. ULBP4 is a monomeric, non-antigen presenting MHC molecule that does not interact with beta 2-microglobulin. It is a ligand for both TCR gamma δ and homodimeric NKG2D, the latter which appears on NK, CD8+ and  gamma δ T cells. NKG2D ligation induces cellular cytotoxicity and cytokine secretion. Mature human ULBP4 is a 234 amino acid (aa) type I transmembrane protein. It contains a 196 aa extracellular region (aa 30-225), and a short 15 aa cytoplasmic domain. Multiple splice variants exist. One shows a five aa substitution for aa 208-263, a second shows a deletion of aa 28-63, a third shows a two aa substitution for aa 208-263, while a fourth possesses an 18 aa substitution for Gly30. Soluble ULBP4 is described to be 35 kDa in size. There is no reported rodent counterpart to ULBP4.

  • Long Name:
    UL16 Binding Protein-4
  • Entrez Gene IDs:
    135250 (Human); 379043 (Mouse); 292461 (Rat)
  • Alternate Names:
    bA350J20.7; LETAL; LETALRAE-1-like transcript 4; Lymphocyte effector toxicity activation ligand; MGC125309; N2DL4; N2DL-4; NKG2D ligand 4; NKG2DL4; RAET1E; RAET1E2; retinoic acid early transcript 1ERL-4; RL-4; ULBP4; ULBP-4; ULBP4MGC125308
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus.
    Authors: Bjorkstrom NK, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, Michaelsson J, Malmberg KJ, Klingstrom J, Ahlm C, Ljunggren HG
    J. Exp. Med., 2011;208(1):13-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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