Detection of Human UNC13D by Western Blot.
Western blot shows lysates of NK‑92 human natural killer lymphoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB89661) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for UNC13D at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
UNC13D in NK‑92 Human Cell Line.
UNC13D was detected in immersion fixed NK‑92 human natural killer lymphoma cell line using Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB89661) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Human UNC13D by Simple WesternTM.
Simple Western lane view shows lysates of NK‑92 human natural killer lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for UNC13D at approximately 112 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB89661). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
UNC13D (also Munc13-4) is a 123 kDa cytoplasmic and
peripheral membrane protein that is expressed at highest levels in hematopoietic
tissues. UNC13D appears to play a role in vesicle maturation during
exocytosis and its expression is obligatory for exocytosis of cytolytic
granules from NK and T cells. A point mutation in intron 1 of UNC13D causes
familial hematophagocytic lymphohistiocytosis type3, a rare autosomal recessive
immune deficiency. The mutation disrupts transcription factor binding and
prevents expression of an alternative isoform that is required for lymphocyte
cytotoxicity. The conventional and alternative isoforms are identical from
amino acids (aa) 40-1090 but have distinct N-terminal segments. Human and mouse have 75% aa sequence identity throughout the region used as the immunogen. It is unknown whether mice express the alternative isoform.
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