Human VIAAT/SLC32A1 Antibody

Catalog # Availability Size / Price Qty
MAB6847
MAB6847-SP

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VIAAT/SLC32A1 in Rat Cortical Stem Cells.
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Human VIAAT/SLC32A1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human VIAAT/SLC32A1 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 731307
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human VIAAT/SLC32A1
Ala2-Phe133
Accession # Q9H598
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry VIAAT/SLC32A1 antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

VIAAT/SLC32A1 in Rat Cortical Stem Cells. VIAAT/SLC32A1 was detected in immersion fixed 7-day differentiated rat cortical stem cells using Mouse Anti-Human VIAAT/SLC32A1 Monoclonal Antibody (Catalog # MAB6847) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to neurons. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VIAAT/SLC32A1

VIAAT (Vesicular inhibitory amino acid transporter; also VGAT and SLC32A1) is a 56-58 kDa member of the amino acid/polyamine transporter 2 family of proteins. It is expressed in inhibitory neurons throughout the CNS, in both resting and activated lymphocytes, and in pancreatic alpha -cells (in rodent). VIAAT is believed to transport both GABA and glycine across synaptic vesicle membranes in an electrical and pH gradient-dependent manner. Once transported, these neurotransmitters are released at the presynaptic membrane, acting as inhibitory factors in the mature nervous system, and excitatory factors in the immature nervous system. Human VIAAT is a 525 amino acid (aa) 10-transmembrane nonglycosylated protein. The N-terminus (aa 1-133) and C-terminus (aa 511-525) are cytoplasmic. There is a 52 kDa short form that is not well characterized. Over aa 2-133, human VIAAT shares 95% aa identity with mouse VIAAT.

Long Name
Vesicular Inhibitory Amino Acid Transporter
Entrez Gene IDs
140679 (Human); 22348 (Mouse); 83612 (Rat)
Alternate Names
bA122O1.1; hVIAAT; SLC32A1; solute carrier family 32 (GABA vesicular transporter), member 1; Solute carrier family 32 member 1; Vesicular GABA transporter; vesicular inhibitory amino acid transporter; VGAT; VGATGABA and glycine transporter; VIAAT

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Human VIAAT/SLC32A1 Antibody
By Anonymous on 11/01/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Cortical stem cells Species: Rat