|VIAAT/SLC32A1 in Rat Cortical Stem Cells. VIAAT/SLC32A1 was detected in immersion fixed 7-day differentiated rat cortical stem cells using Mouse Anti-Human VIAAT/SLC32A1 Monoclonal Antibody (Catalog # MAB6847) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to neurons. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.|
VIAAT (Vesicular inhibitory amino acid transporter; also VGAT and SLC32A1) is a 56-58 kDa member of the amino acid/polyamine transporter 2 family of proteins. It is expressed in inhibitory neurons throughout the CNS, in both resting and activated lymphocytes, and in pancreatic alpha -cells (in rodent). VIAAT is believed to transport both GABA and glycine across synaptic vesicle membranes in an electrical and pH gradient-dependent manner. Once transported, these neurotransmitters are released at the presynaptic membrane, acting as inhibitory factors in the mature nervous system, and excitatory factors in the immature nervous system. Human VIAAT is a 525 amino acid (aa) 10-transmembrane nonglycosylated protein. The N-terminus (aa 1-133) and C-terminus (aa 511-525) are cytoplasmic. There is a 52 kDa short form that is not well characterized. Over aa 2-133, human VIAAT shares 95% aa identity with mouse VIAAT.
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