Human vWF-A2 (ADAMTS13-cleaved) Antibody

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Product Details
Citations (1)
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Human vWF-A2 (ADAMTS13-cleaved) Antibody Summary

Species Reactivity
Detects human vWF‑A2 (ADAMTS13-cleaved) in Western blots. This antibody detects a nascent epitope that is generated upon cleavage of recombinant human (rh) vWF-A2 domain with ADAMTS13. It does not detect intact rhvWF-A2 domain.
Monoclonal Mouse IgG1 Clone # 490628
Protein A or G purified from hybridoma culture supernatant
KLH-conjugated human vWF synthetic peptide
Accession # NP_000543
Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human vWF-A2 (Catalog # 2764-WF) cleaved with Recombinant Human ADAMTS13 (Catalog # 4245-AD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: vWF-A2

von Willebrand Factor (vWF) is a large, multimeric glycoprotein made by endothelial cells and megakaryocytes. The pre-pro-vWF protein contains 2813 amino acids (aa), which consists of 22 aa signal peptide, 741 aa propeptide, and mature vWF monomer of 2050 aa (1-4). The pro-vWF undergoes dimerization in the endoplasmic reticulum (ER) through C-terminal “cysteine-knot” (CK) domain. The pro-vWF dimmers are transported to Golgi and form multimers by forming disulfide bond in amino‑terminal region of the mature form. The proteolytic processing of pro-region also occurs in Golgi. The matured vWF is stored in Weibel-Pallade bodies in endothelial cells and granules in megakaryocytes and platelets. The unusually-large vWF (ulvWF) multimers released from cells are very efficient in binding to platelets to form thrombus. The population of these highly active ulvWF multimers is controlled by a specific protease, ADAMTS13, which cleaves between residues Tyr1605 and Met1606 in the A2 domain of vWF. In the plasma, vWF appears as a series of large and intermediate multimers with molecular masses from several thousand to 500 kDa. vWF also performs hemostatic functions (3-5). In a high shear-stressed environment, vWF undergoes conformational change to expose a binding site for glycoprotein Ib alpha. As a result, vWF facilitates aggregation of platelets. In addition to platelet binding, vWF binds coagulation factor VIII to increase the lifetime of FVIII in plasma. The purified rhvWF-A2 contains the A2 domain of vWF.

  1. Sadler, J.E. (1998) Annu. Rev. Biochem. 67:395.
  2. Ruggeri, Z.M. (2003) Cur. Opin. Hemat. 10:142.
  3. Michiels, J.J. et al. (2006) Clin. Appl. Thromb. Hemost. 12:397.
  4. Groot, E. et al. (2007) Cur. Opin. Hemat. 14:284.
  5. Lenting, P.J. et al. (2007) J. Thromb. Haemos. 5:1353.
Long Name
von Willebrand Factor A2 Domain
Entrez Gene IDs
7450 (Human); 22371 (Mouse); 116669 (Rat)
Alternate Names
F8VWF; VWD; VWF von Willebrand factor; vWFA2; vWF-A2

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Citation for Human vWF-A2 (ADAMTS13-cleaved) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A novel calcium-binding site of von Willebrand factor A2 domain regulates its cleavage by ADAMTS13.
    Authors: Zhou M, Dong X, Baldauf C, Chen H, Zhou Y, Springer TA, Luo X, Zhong C, Grater F, Ding J
    Blood, 2011;117(17):4623-31.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot


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