Human WASP Antibody

Catalog # Availability Size / Price Qty
MAB3070
MAB3070-SP
Human WASP Antibody in Data
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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human WASP Antibody Summary

Species Reactivity
Human
Specificity
Detects human WASP in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 336925
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human WASP
Lys230-Gln310
Accession # P42768
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human WASP
Immunocytochemistry
8-25 µg/mL
Immersion fixed U937 human histiocytic lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry View Larger

WASP in U937 Human Cell Line. WASP was detected in immersion fixed U937 human histiocytic lymphoma cell line using Mouse Anti-Human WASP Monoclonal Antibody (Catalog # MAB3070) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WASP

WASP is a 54kDa protein belonging to the WASP/SCAR/WAVE family of proteins involved in actin polymerization. It is a highly conserved protein and is expressed selectively in hematopoietic stem cell-derived lineages. WASP contains multiple domains with unique functions. Mutations in WASP are associated with diseases of the hematopoietic/immunologic system.

Long Name
Wiskott-Aldrich Syndrome Protein
Entrez Gene IDs
7454 (Human); 22376 (Mouse)
Alternate Names
IMD2THC; Thrombocytopenia 1; WAS; WASP; WiskTHC1; wiskthrombocytopenia 1 (X-linked)

Product Datasheets

Citation for Human WASP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Effect of ovarian steroids on gene expression profile in human uterine microvascular endothelial cells.
    Authors: Yasuo T, Kitaya K
    Fertil. Steril., 2009;92(2):709-21.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human WASP Antibody

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Human WASP Antibody
By Anonymous on 12/07/2020
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma