Human WASP Antibody

Catalog # Availability Size / Price Qty
WASP in U937 Human Cell Line.
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Product Details
Citations (1)
Supplemental Products
Reviews (2)

Human WASP Antibody Summary

Species Reactivity
Detects human WASP in direct ELISAs and Western blots.
Monoclonal Mouse IgG1 Clone # 336925
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human WASP
Accession # P42768
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human WASP
8-25 µg/mL
Immersion fixed U937 human histiocytic lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

WASP in U937 Human Cell Line. WASP was detected in immersion fixed U937 human histiocytic lymphoma cell line using Mouse Anti-Human WASP Monoclonal Antibody (Catalog # MAB3070) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WASP

WASP is a 54kDa protein belonging to the WASP/SCAR/WAVE family of proteins involved in actin polymerization. It is a highly conserved protein and is expressed selectively in hematopoietic stem cell-derived lineages. WASP contains multiple domains with unique functions. Mutations in WASP are associated with diseases of the hematopoietic/immunologic system.

Long Name
Wiskott-Aldrich Syndrome Protein
Entrez Gene IDs
7454 (Human); 22376 (Mouse)
Alternate Names
IMD2THC; Thrombocytopenia 1; WAS; WASP; WiskTHC1; wiskthrombocytopenia 1 (X-linked)

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Citation for Human WASP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Effect of ovarian steroids on gene expression profile in human uterine microvascular endothelial cells.
    Authors: Yasuo T, Kitaya K
    Fertil. Steril., 2009;92(2):709-21.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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Reviews for Human WASP Antibody

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Human WASP Antibody
By Anonymous on 03/30/2022
Application: ICC/IF Sample Tested: U937 human histiocytic lymphoma cell line Species: Human

Human WASP Antibody
By Anonymous on 12/07/2020
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma