Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 336925
View all WASP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human WASP
Lys230-Gln310
Accession # P42768

Specificity

Detects human WASP in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human WASP Antibody

WASP in U937 Human Cell Line.

WASP was detected in immersion fixed U937 human histiocytic lymphoma cell line using Mouse Anti-Human WASP Monoclonal Antibody (Catalog # MAB3070) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human WASP Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human WASP

Reviewed Applications

Read 2 reviews rated 4.5 using MAB3070 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: WASP

WASP is a 54kDa protein belonging to the WASP/SCAR/WAVE family of proteins involved in actin polymerization. It is a highly conserved protein and is expressed selectively in hematopoietic stem cell-derived lineages. WASP contains multiple domains with unique functions. Mutations in WASP are associated with diseases of the hematopoietic/immunologic system.

Long Name

Wiskott-Aldrich Syndrome Protein

Alternate Names

Thrombocytopenia 1, WAS

Entrez Gene IDs

7454 (Human); 22376 (Mouse)

Gene Symbol

WAS

UniProt

P42768

Additional WASP Products

Product Documents for Human WASP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human WASP Antibody

For research use only

Citations for Human WASP Antibody

Customer Reviews for Human WASP Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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3 Stars
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2 Stars
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1 Stars
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Customer Images

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  • Human WASP Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: U937 human histiocytic lymphoma cell line
    Species: Human
    Verified Customer | Posted 03/30/2022
    Human WASP Antibody MAB3070
  • Human WASP Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human WASP Antibody MAB3070

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Protocols

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FAQs

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