Human WIF-1 Antibody

Catalog # Availability Size / Price Qty
MAB134-050
MAB134-500
MAB134-SP
WIF‑1 in Human Breast.
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Product Details
Citations (3)
FAQs
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Human WIF-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human WIF-1 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse WIF‑1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 133015
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human WIF-1
Gly29-Trp379
Accession # AAD25402
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human WIF‑1 (Catalog # 1341-WF)
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry WIF‑1 antibody in Human Breast by Immunohistochemistry (IHC-P). View Larger

WIF‑1 in Human Breast. WIF‑1 was detected in immersion fixed paraffin-embedded sections of human breast using 8 µg/mL Mouse Anti-Human WIF‑1 Monoclonal Antibody (Catalog # MAB134) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WIF-1

WIF-1 is a secreted protein that binds Wnt proteins and antagonizes Wnt-induced signaling. WIF-1 expression is down regulated in several human cancers.

Long Name
Wnt Inhibitory Factor
Entrez Gene IDs
11197 (Human); 24117 (Mouse)
Alternate Names
WIF1; WIF-1; WNT inhibitory factor 1

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Citations for Human WIF-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Evidence for altered Wnt signaling in psoriatic skin.
    Authors: Gudjonsson JE, Johnston A, Stoll SW
    J. Invest. Dermatol., 2010-04-08;130(7):1849-59.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. Functional significance of Wnt inhibitory factor-1 gene in kidney cancer.
    Authors: Kawakami K, Hirata H, Yamamura S, Kikuno N, Saini S, Majid S, Tanaka Y, Kawamoto K, Enokida H, Nakagawa M, Dahiya R
    Cancer Res., 2009-11-03;69(22):8603-10.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Wnt pathway inhibitors are strongly down-regulated in pituitary tumors.
    Authors: Elston MS, Gill AJ, Conaglen JV, Clarkson A, Shaw JM, Law AJ, Cook RJ, Little NS, Clifton-Bligh RJ, Robinson BG, McDonald KL
    Endocrinology, 2007-12-13;149(3):1235-42.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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