Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 133015
View all WIF-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human WIF-1
Gly29-Trp379
Accession # AAD25402

Specificity

Detects human WIF-1 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse WIF‑1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human WIF‑1 Antibody

WIF‑1 antibody in Human Breast by Immunohistochemistry (IHC-P).

WIF‑1 in Human Breast.

WIF‑1 was detected in immersion fixed paraffin-embedded sections of human breast using 8 µg/mL Mouse Anti-Human WIF‑1 Monoclonal Antibody (Catalog # MAB134) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human WIF‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast

Western Blot

1 µg/mL
Sample: Recombinant Human WIF‑1 (Catalog # 1341-WF)

Reviewed Applications

Read 1 review rated 4 using MAB134 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: WIF-1

WIF-1 is a secreted protein that binds Wnt proteins and antagonizes Wnt-induced signaling. WIF-1 expression is down regulated in several human cancers.

Long Name

Wnt Inhibitory Factor

Alternate Names

WIF1

Entrez Gene IDs

11197 (Human); 24117 (Mouse)

Gene Symbol

WIF1

UniProt

AAD25402

Additional WIF-1 Products

Product Documents for Human WIF‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human WIF‑1 Antibody

For research use only

Citations for Human WIF‑1 Antibody

Customer Reviews for Human WIF‑1 Antibody (1)

4 out of 5
1 Customer Rating
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  • Human WIF-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HCT116 cell line lysate
    Species: Human
    Verified Customer | Posted 02/19/2019
    Expression of 42KDa human WIF1 in HCT116 cells transfected with WIF1 expression vector for 24 hours.
    Human HCT116 cells transfected with WIF1 expression vector for 24 hours.
    Human WIF‑1 Antibody MAB134

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Protocols

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FAQs

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