Human ZEB1 Antibody

Catalog # Availability Size / Price Qty
MAB6708
MAB6708-SP
ZEB1 in MDA‑MB‑231 Human Cell Line.
2 Images
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (2)

Human ZEB1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ZEB1 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 639914
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ZEB1
Glu430-Ser575
Accession # P37275
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry ZEB1 antibody in MDA-MB-231 Human Cell Line by Immunocytochemistry (ICC). View Larger

ZEB1 in MDA‑MB‑231 Human Cell Line. ZEB1 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Mouse Anti-Human ZEB1 Monoclonal Antibody (Catalog # MAB6708) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow Cytometry Detection of ZEB-1 antibody in MDA-MB-231 Human Cell Line antibody by Flow Cytometry View Larger

Detection of ZEB-1 in MDA‑MB‑231 Human Cell Line by Flow Cytometry MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human ZEB1 Monoclonal Antibody (Catalog # MAB6708, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZEB1

Zinc finger E-box-binding homeobox 1 (ZEB1; also transcription factor 8 (TCF-8)) is a 124 kDa member of the delta-EF1/ZFH-1 C2H2-type zinc finger family.  Human ZEB1 is 1124 amino acids (aa) in length. The protein contains seven C2H2-type zinc fingers and one homeobox DNA-binding domain. In addition, there are eight phosphoserines and one phosphothreonine. Residues 989-1124 make up a glutamine-rich area. Within aa 430-575, human ZEB1 shares 84% and 82% aa sequence identity with mouse and rat ZEB1, respectively. The protein is expressed in heart and skeletal muscle, and defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3, a rare disease involving metaplasia and overgrowth of the corneal endothelial cells.

Long Name
Zinc Finger E-box Binding Homeobox 1
Entrez Gene IDs
6935 (Human); 21417 (Mouse); 25705 (Rat)
Alternate Names
AREB6; AREB6MGC133261; BZP; delta-crystallin enhancer binding factor 1; DELTAEF1; FECD6; Negative regulator of IL2; NIL-2-A zinc finger protein; NIL2A; NIL-2-A; posterior polymorphous corneal dystrophy 3; PPCD3; TCF8; TCF-8; TCF8BZP; transcription factor 8 (represses interleukin 2 expression); Transcription factor 8; ZEB; ZEB1; ZFHEP; ZFHX1A; zinc finger E-box binding homeobox 1; zinc finger E-box-binding homeobox 1; zinc finger homeodomain enhancer-binding protein

Product Datasheets

Citations for Human ZEB1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. EMT transcription factor ZEB1 alters the epigenetic landscape of colorectal cancer cells
    Authors: P Lindner, S Paul, M Eckstein, C Hampel, JK Muenzner, K Erlenbach-, HP Ahmed, V Mahadevan, T Brabletz, A Hartmann, J Vera, R Schneider-
    Cell Death Dis, 2020;11(2):147.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Plasma Treatment Limits Human Melanoma Spheroid Growth and Metastasis Independent of the Ambient Gas Composition
    Authors: S Hasse, T Meder, E Freund, T von Woedtk, S Bekeschus
    Cancers (Basel), 2020;12(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human ZEB1 Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human ZEB1 Antibody
By Anonymous on 06/27/2018
Application: Flow Sample Tested: Pancreas tissue Species: Human

Human ZEB1 Antibody
By Anonymous on 11/17/2017
Application: ICC/IF Sample Tested: Urinary bladder transitional cell carcinoma cell line Species: Human