ZEB1 in MDA‑MB‑231 Human Cell Line. ZEB1 was detected in immersion fixed MDA‑MB‑231 human breast cancer cell line using Mouse Anti-Human ZEB1 Monoclonal Antibody (Catalog # MAB6708) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of ZEB-1 in MDA‑MB‑231 Human Cell Line by Flow Cytometry MDA‑MB‑231 human breast cancer cell line was stained with Mouse Anti-Human ZEB1 Monoclonal Antibody (Catalog # MAB6708, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Zinc finger E-box-binding homeobox 1 (ZEB1; also
transcription factor 8 (TCF-8)) is a 124 kDa member of the delta-EF1/ZFH-1
C2H2-type zinc finger family. Human ZEB1
is 1124 amino acids (aa) in length. The protein contains seven C2H2-type zinc
fingers and one homeobox DNA-binding domain. In addition, there are eight phosphoserines and one
phosphothreonine. Residues 989-1124 make
up a glutamine-rich area. Within aa 430-575, human ZEB1
shares 84% and 82% aa sequence identity with mouse and rat ZEB1, respectively. The protein is expressed in heart and
skeletal muscle, and defects in ZEB1 are the cause of posterior polymorphous
corneal dystrophy type 3, a rare disease involving metaplasia and overgrowth of
the corneal endothelial cells.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.