Human ZEB1 Antibody Summary
Accession # P37275
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
ZEB1 in MDA‑MB‑231 Human Cell Line. ZEB1 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Mouse Anti-Human ZEB1 Monoclonal Antibody (Catalog # MAB6708) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of ZEB-1 in MDA‑MB‑231 Human Cell Line by Flow Cytometry MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human ZEB1 Monoclonal Antibody (Catalog # MAB6708, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Zinc finger E-box-binding homeobox 1 (ZEB1; also transcription factor 8 (TCF-8)) is a 124 kDa member of the delta-EF1/ZFH-1 C2H2-type zinc finger family. Human ZEB1 is 1124 amino acids (aa) in length. The protein contains seven C2H2-type zinc fingers and one homeobox DNA-binding domain. In addition, there are eight phosphoserines and one phosphothreonine. Residues 989-1124 make up a glutamine-rich area. Within aa 430-575, human ZEB1 shares 84% and 82% aa sequence identity with mouse and rat ZEB1, respectively. The protein is expressed in heart and skeletal muscle, and defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3, a rare disease involving metaplasia and overgrowth of the corneal endothelial cells.
Citations for Human ZEB1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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EMT transcription factor ZEB1 alters the epigenetic landscape of colorectal cancer cells
Authors: P Lindner, S Paul, M Eckstein, C Hampel, JK Muenzner, K Erlenbach-, HP Ahmed, V Mahadevan, T Brabletz, A Hartmann, J Vera, R Schneider-
Cell Death Dis, 2020;11(2):147.
Sample Types: Whole Cells
Plasma Treatment Limits Human Melanoma Spheroid Growth and Metastasis Independent of the Ambient Gas Composition
Authors: S Hasse, T Meder, E Freund, T von Woedtk, S Bekeschus
Cancers (Basel), 2020;12(9):.
Sample Types: Whole Cells
Applications: Flow Cytometry
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