IL-6 Antibody (AP-MAB0847) - Azide and BSA Free
Novus Biologicals | Catalog # NBP1-06663
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Format
Product Specifications
Immunogen
Clonality
Host
Isotype
Applications for IL-6 Antibody (AP-MAB0847) - Azide and BSA Free
Flow Cytometry
Immunohistochemistry
Immunohistochemistry-Frozen
Immunoprecipitation
Extremely low level of LPS (< 0.002EU/ug IgG).
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Formulation
Format
Preservative
Concentration
Shipping
Stability & Storage
Background: IL-6
Long Name
Alternate Names
Gene Symbol
Additional IL-6 Products
Product Documents for IL-6 Antibody (AP-MAB0847) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IL-6 Antibody (AP-MAB0847) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for IL-6 Antibody (AP-MAB0847) - Azide and BSA Free
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Q: For the IL-6 antibody (NBP1-06663) made in rat, what antigen retrieval I should use?
A:
This antibody has only been validated in IHC-Fr. If using this in IHC-P, we are not sure it will work. Since IL-6 is a secretory protein and can briefly exist inside the cells Uniprot, IHC indeed can detect its signals Protien Atlas. Although the above protein atlas website is for human proteins, similarity among high mammals renders that mouse IL-6 can be detected with HIER pH6 (citrate buffer) if this antibody is used in IHC-P (the antigen retrieval methods are heavily depending on the biological/physiological/histological properties of the protein; irrelevant to the antibody).
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Q: I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
A:
We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.
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Q: For the IL-6 antibody (NBP1-06663) made in rat, what antigen retrieval I should use?
A:
This antibody has only been validated in IHC-Fr. If using this in IHC-P, we are not sure it will work. Since IL-6 is a secretory protein and can briefly exist inside the cells Uniprot, IHC indeed can detect its signals Protien Atlas. Although the above protein atlas website is for human proteins, similarity among high mammals renders that mouse IL-6 can be detected with HIER pH6 (citrate buffer) if this antibody is used in IHC-P (the antigen retrieval methods are heavily depending on the biological/physiological/histological properties of the protein; irrelevant to the antibody).
-
Q: I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
A:
We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.
Associated Pathways
Dendritic Cell Lineage Development Pathways
Embryonic and Induced Pluripotent Stem Cell Differentiation Pathways & Lineage-specific Markers
Hematopoietic Stem Cell Differentiation Pathways & Lineage-specific Markers
IL-9 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
Jak/STAT Signaling Pathway
Mesenchymal Stem Cell Differentiation Pathways & Lineage-specific Markers
NOD-like Receptor Signaling Pathways
Th17 Differentiation Pathway
Toll-Like Receptor Signaling Pathways
