MEK1 Antibody (OTI1F5) - Azide and BSA Free

Name: MEK1 Antibody (OTI1F5) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-72643
Price: 599 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-72643

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # OTI1F5

Format

Azide and BSA Free

View all MEK1 Primary Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human MAP2K1 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

43.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MEK1 Antibody (OTI1F5) - Azide and BSA Free

Western Blot: MEK1 Antibody (OTI1F5)Azide and BSA Free [NBP2-72643]

Western Blot: MEK1 Antibody (OTI1F5) - Azide and BSA Free [NBP2-72643] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEK1.

Immunocytochemistry/ Immunofluorescence: MEK1 Antibody (OTI1F5) - Azide and BSA Free [NBP2-72643]

Immunocytochemistry/Immunofluorescence: MEK1 Antibody (OTI1F5) - Azide and BSA Free [NBP2-72643] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEK1.

Immunohistochemistry: MEK1 Antibody (OTI1F5) - Azide and BSA Free [NBP2-72643]

Immunohistochemistry: MEK1 Antibody (OTI1F5) - Azide and BSA Free [NBP2-72643] - Staining of paraffin-embedded pancreas tissue using anti-MEK1mouse monoclonal antibody.

Immunohistochemistry: MEK1 Antibody (OTI1F5) - Azide and BSA Free [NBP2-72643]

Immunohistochemistry: MEK1 Antibody (OTI1F5) - Azide and BSA Free [NBP2-72643] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-MEK1 mouse monoclonal antibody.

Immunohistochemistry: MEK1 Antibody (OTI1F5) - Azide and BSA Free [NBP2-72643]

Immunohistochemistry: MEK1 Antibody (OTI1F5) - Azide and BSA Free [NBP2-72643] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-MEK1 mouse monoclonal antibody.

Immunohistochemistry: MEK1 Antibody (OTI1F5) - Azide and BSA Free [NBP2-72643]

Immunohistochemistry: MEK1 Antibody (OTI1F5) - Azide and BSA Free [NBP2-72643] - Staining of paraffin-embedded colon tissue using anti-MEK1 mouse monoclonal antibody.

Immunohistochemistry: MEK1 Antibody (OTI1F5) - Azide and BSA Free [NBP2-72643]

Immunohistochemistry: MEK1 Antibody (OTI1F5) - Azide and BSA Free [NBP2-72643] - Staining of paraffin-embedded endometrium tissue using anti-MEK1 mouse monoclonal antibody.

Immunohistochemistry: MEK1 Antibody (OTI1F5) - Azide and BSA Free [NBP2-72643]

Immunohistochemistry: MEK1 Antibody (OTI1F5) - Azide and BSA Free [NBP2-72643] - Staining of paraffin-embedded Ovary tissue using anti-MEK1 mouse monoclonal antibody.

Immunohistochemistry: MEK1 Antibody (OTI1F5) - Azide and BSA Free [NBP2-72643]

Immunohistochemistry: MEK1 Antibody (OTI1F5) - Azide and BSA Free [NBP2-72643] - Staining of paraffin-embedded prostate tissue using anti-MEK1 mouse monoclonal antibody.

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Applications for MEK1 Antibody (OTI1F5) - Azide and BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: MEK1

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992) Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).

Long Name

Mitogen-activated Protein Kinase Kinase 1

Alternate Names

MAP2K1, MKK1

Gene Symbol

MAP2K1

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Product Specific Notices for MEK1 Antibody (OTI1F5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.